Polysaccharide HPLC & SFC Columns: CHIRAL ART

In this whitepaper, YMC Europe provides useful information for all chromatographers who deal with the analysis and/or purification of oligonucleotides. Therapeutic oligonucleotides (TOs) are synthetic DNA or RNA oligomers designed to target specific genes or transcriptional pathways with the purpose of treating a wide range of diseases, including cancer, genetic disorders, and viral infections. The focus of this whitepaper is on anion exchange chromatography (AEX) and ion pair reversed phase chromatography (IP-RP). For both modes, the main aspects are the selection of a suitable stationary and mobile phase as well as the optimum temperature.

This application note presents the rapid analysis of pesticides used on a golf course, the simultaneous separation of pesticides by LC/MS, and method transfer from HPLC to UHPLC.

Until now, there have been 5 different chiral stationary phases available from YMC which can cover more than 90% of all chiral racemic separations. The latest addition to the YMC chiral toolkit is CHIRAL ART Cellulose-SJ. This new immobilised chiral selector offers unique selectivity which complements the other members of the family of YMC chiral phases.

The analysis of the original composition of plant material is necessary for such purposes as phenotype determination and quality control of medical cannabis used for therapeutic treatment. HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. In this application note two fast methods are presented achieving high-performance in less than 9 min.

Tebuconazole is a chiral racemic fungicide used as a pesticide as well as a wood preservative. Bayer AG (Crop Science Division), the manufacturer of this agent, was looking for an effective LC-MS compatible method for separating Tebuconazole. This application note presents a method of analysis including a reversed phase separation mode and a mobile phase containing acid for ionization.

In this application note, a success story with CHIRAL ART from phase screening, performed by YMC Europe GmbH in Dinslaken, to a published method for the enantioselective determination of the two β-blockers metoprolol and propranolol with CHIRAL ART Cellulose-SB in complex sample matrices like human plasma and saliva is presented.

The enantioselective separation of chiral basic substances is of high interest especially in the pharmaceutical sector, as many pharmaceuticals are chiral bases. Often, one enantiomer shows higher pharmacological activity. The other one may be inactive or even toxic. More and more chiral separations are being performed in SFC mode, but there still remain some challenges for basic compounds. This application note describes the use of CHIRAL ART Amylose-SA for the determination and separation of 27 biologically active basic compounds.

This application note demonstrates a method for the LC-based chiral separation of spiroxamine that can be coupled to LC-MS/MS in the background of analyses of residues of spiroxamine at trace concentrations. This method was developed by YMC using a CHIRAL ART Amylose-SA column using water, ethanol and diethylamine.

Immobilized CHIRAL ART columns from YMC can be used in both: normal phase and reverse phase mode, as they are stable to non-aqueous and aqueous eluents. Working in reversed phase mode is beneficial especially when applying hydrophilic compounds with limited solubility in organic solvents. This application note demonstrates the optimization of a chiral separation method, using warfarin as an example.

Metalaxyl is a fungicide used to fight plant diseases (downey mildew) in agricultural applications. Metalaxyl is a racemic phenylamide, with two enantiomers. (R)-Metalaxyl has a higher activity (Metalaxyl-M, Mefenoxam). In Germany, Switzerland and Austria only the application of this active enantiomer is permitted. The valid determination and quantification of Metalaxyl residues is very important for monitoring food, soils and ground water. This application note demonstrates the use of CHIRAL ART Cellulose-SB from YMC to separate the enantiomers of Metalaxyl.