PhyTip® Columns and MiniPrep PhyTip® Columns

A new synergy in antibody purification combines advanced chromatography with high-performance resin technology to deliver yield, throughput, and scalability.

Discover how Biotage's PrismA PhyTip® columns leverage the power of patented dual flow chromatography alongside Cytiva’s MabSelect PrismA™ resin, creating a uniquely effective solution. This synergistic pairing enables high-throughput, small-volume, automated purification while benefiting from the superior binding capacity of the PrismA resin.

The introduction of recombinant proteins that poses amino acid-tagged sites has resulted in a mechanism for many researchers to specifically extract proteins of interest from a mass of unwanted macromolecules in order to study the protein’s structure and function. However, the isolation of recombinant proteins and antibodies for analysis by any of today’s high sensitivity detection techniques such as mass spectrometry or protein biochips involves a tedious and time consuming macro-scale process. In addition these same isolation processes result in an end product that may not retain functional integrity and therefore the end result could be compromised. In this application note read how PhyNexus, Inc. has developed a unique system to overcome the issue of producing sufficient quantities of purified and fully functional protein at a micro-volume scale.

The PhyNexus PhyTip columns are innovative purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. One area with an immediate need for high throughput, automated sample preparation is antibody leads screening. In this application note read how the Tecan Freedom EVO can be used in conjunction with PhyTip columns to purify 96 hybridoma supernatants in as little as 15 minutes.

PhyNexus PhyTip columns are innovative purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. Key to the success of these purification tools is the design of the mechanism to retain the affinity resin bed, with minimum dead volume and maximum capture potential. In this application note read how the use of PhyTip columns, in conjunction with a Freedom EVO liquid handling platforms and Multichannel Arm™ 96, offers a high throughput automated solution for sample preparation and protein purification.

The rapidly growing field of antibody and protein engineering continues to create the demand for technologies that increase the productivity and throughput of protein interaction analysis. This application note describes a unique yet simple automated process to purify and enrich antibodies and proteins using PhyTip Columns and their subsequent quantification using a microfluidic LabChip device.

In this application note it is demonstrated that the PhyTip 5K gel filtration columns and the MEA Personal Purification System represent a high throughput solution for rapid desalting and buffer exchange. PhyTip 5K gel filtration columns can process 12 samples in 30 minutes, which is significantly faster compared to the laborious and time consuming dialysis procedure.

Advances in high-resolution separation methods such as HPLC and CE are a good solution for identification, quantification and profiling of glycans. Though these analytical tools are excellent separation methods, the detection of glycans poses a challenge because of the lack of a natural chromophore. Labeling strategies circumvent this problem by providing a simple and efficient tagging procedure. Recent advances in sample cleanup using PhyTip columns containing normal phase resin allow users to reproducibly process samples in a high-throughput fashion.

A common problem in the purification of proteins expressed at low concentration is a resulting pure protein that is too dilute for subsequent assays. This application note demonstrates how the PhyNexus two-step purification method, which utilizes PhyTip columns, is suitable for obtaining a desirable yield and purity. The efficiency of elution from a PhyTip column, combined with retention of protein activity, provide a good solution for an automatable concentration process.

The PhyNexus PhyTip® columns are purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. This application note demonstrates how the PhyTip ProPlus columns are ideal for antibody screening from hybridoma supernatants or for high throughput extraction and purification of expressed antibodies.

The PhyNexus PhyTip® columns from PhyNexus enable the capture, purification and enrichment of proteins from a variety of sources. Key to the success of these purification tools is the design of the mechanism to retain the affinity resin bed, with minimum dead volume and maximum capture potential. In this application note a method is presented for the purification of IgG from various sources. It is demonstrated that PhyTip columns have extremely high binding capacity and can bind greater than 100 μg IgG, and can also efficiently recover as little as 200 ng of IgG.

The Tip Concentrating Effect is a process that allows the recovery of high concentrations of proteins in a single purification procedure. PhyTip pipette tip columns have been designed to produce very high concentrations of recovered proteins compared to competitive techniques such as spin columns, gravity columns, magnetic beads and FPLC. The Tip Concentrating Effect relies on the ability of PhyTip columns to capture protein on very small column bed volumes. Then proteins are recovered in a highly efficient and effective process. All conditions being equal, with any two different resin bed sizes of PhyTip columns, the smaller resin bed size will produce the highest concentration of recovered protein.

In life science research, it is often necessary to isolate specific proteins from natural sources in order to study them. A method commonly used for this is immunoprecipitation (IP). In IP, researchers use antibodies to bind to a protein of interest. These antibody-protein complexes are isolated from the complex sample using protein A (ProA) or protein G (ProG) affinity resin. After washing nonspecifically bound proteins away, the antibody-protein complex is eluted from the resin prior to further analysis. High Performance Immunoprecipitation (HPIP) enables the process to be easily automated for high throughput using PhyTip® purification columns and robotic liquid handling systems.