The Phynex PhyTip Column Method for Obtaining High Concentrations of Active Proteins
13 Feb 2013A common problem in the purification of proteins expressed at low concentration is a resulting pure protein that is too dilute for subsequent assays. This application note demonstrates how the PhyNexus two-step purification method, which utilizes PhyTip columns, is suitable for obtaining a desirable yield and purity. The efficiency of elution from a PhyTip column, combined with retention of protein activity, provide a good solution for an automatable concentration process.
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PhyTip® Columns and MiniPrep PhyTip® Columns
BiotagePhyTip® columns are the gold standard for high-throughput, small-scale purification of proteins, antibodies, plasmids, and viral vectors. By integrating advanced chromatography directly into a pipette tip, PhyTip® columns eliminate manual bottlenecks and deliver high quality purification with true "walk-away" automation.