Biotage launches new automated platform for plasmid DNA purification
22 Jun 2021
Biotage has announced the launch of Biotage® PhyPrep, a new automated platform for plasmid DNA purification that releases laboratory staff from the tedium of repetitive, manual labor. The technology takes full advantage of the PhyTip® columns’ dual flow chromatography technology, to yield supercoiled, endotoxin-free, transfection-grade plasmid DNA.
Plasmid DNA, or simply plasmids, serve as important tools in life science and biotechnology industry and academic laboratories. Plasmids are commonly used to clone and amplify (make many copies of) or express particular genes. A wide variety of plasmids are commercially available for such uses. Plasmids are commonly being used in drug discovery and development for biopharmaceuticals. The most common used applications for plasmids within the Biopharmaceutical space are bacterial vector Cloning, Protein Production, Gene Therapy and different Disease Models, which is in line with Biotage’s HumanKind Unlimited focus.
With the new Biotage® PhyPrep platform, the automated process purifying plasmid DNA reduces the risk of human error, and with each sample being handled in the same way, the plasmid yield and purities are more consistent. Many Biopharma laboratories currently outsource plasmid purifications - those laboratories can now bring that work in-house, improving process control, project deliverability while at the same time reducing costs.
Biotage® PhyPrep is the first automated solution for plasmid purification in Maxi, Mega and Giga scales. The various scales differ in the volume of cell culture to be purified, with desired yields ranging from 1 mg to 10 mg of plasmid DNA.
“The launch of the Biotage® PhyPrep will assist laboratories in discovering new therapies for patients, utilizing biological drug molecules such as antibodies and novel gene therapies. A perfect match with the Biotage ethos of “HumanKind Unlimited” – sustainably releasing the potential of science to address the issues faced by global society, making the world healthier, greener and cleaner” states Tomas Blomquist, CEO Biotage.
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Biotage Miniprep PhyTip Columns for Plasmid DNA Purification
BiotageLysate Direct PhyTip® Column Technology consists of novel purification columns that are composed of a pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration, lysate direct PhyTip columns for plasmid DNA purification enable utilization of liquid handing automation found in high throughput laboratories to easily purify up to 96 samples in parallel. In addition, lysate direct PhyTip® columns are also compatible with the MEA Personal Purification System available from PhyNexus for 12-at-a-time sample processing. Features include: No filtration or centrifugation of lysate required Consistent purification of up to 15 μg Transfection grade and sequencing-ready 96 samples at a time Suitable for transfection, transformation, sequencing, PCR and cloning Fast and fully automated from the cell pellet, no lysate centrifugation required
PhyTip® Columns and MiniPrep PhyTip® Columns
BiotagePhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.