Biotage® Horizon 3100
Automated oil and grease extraction for wastewater, providing reliable results with less solvent and safer operation.
Great product, somewhat expensive. Good investment if you are performing > 300 samples per year.
Fats Oils and Grease analysis
Have used Biotage (formally Horizon) equipment for 20 years. Equipment is very reliable and long lasting with regular PMs.
Review Date: 17 Nov 2025 | Biotage
Manufacturer's Response
We sincerely appreciate your review and your long-term partnership over the past two decades. It’s great to hear that the Biotage® Horizon 3100 has remained consistently reliable with regular PMs. Your continued confidence in Biotage equipment means a great deal to us.
Quite a good investment
Oil and Grease analyses
Result for the oil and grease analyses have become more accurate since we bought the instrument and analysts' work more trustworthy.
Review Date: 16 Jul 2022 | Biotage
The time savings have made it a great investment.
US EPA Method 1664B
This instrument has allowed us to increase our throughput for grease and oil analysis in industrial waste water analysis. The time savings have been a great investment and a stress-reliever.
Review Date: 12 Mar 2020 | Biotage
Give it an 8 on a 1-10
Refinery lab
Been a good instrument. Might be nice if the 3 stations were plug and play per se, so could send in just a single station for repair if it went down, instead of the whole unit.
Review Date: 2 Nov 2018 | Biotage
It's the best product on the market for automated SPE for oil and grease extraction.
wastewater analysis of oil and grease epa method 1664b
Results are reproducible but consistently lower than 95% recovery. Sometimes QC passes, but barely.
Review Date: 2 Nov 2018 | Biotage
Works well, only complaint is changing O-rings and the websites difficulty.
Analysis of Wastewater samples
The instrument itself works as it should however we do have to frequently change out the O-rings that surround the solvent inlets. Also when trying to find part numbers, the website is not easy to navigate.
Review Date: 18 Oct 2018 | Biotage
The 3100 is a significant upgrade to an already well-designed workhorse
Oil & Grease/ TPH analysis of wastewater
Our laboratory has used several Horizon extractors over the last 10+ years. The 3100 provides several highly-appreciated upgrades to what was already a well-designed extractor, including an overflow detector, reduced number of solvent reservoirs, and more secure sample holders.
Review Date: 23 Dec 2016 | Biotage
Two Thumbs Up
FOG/TPH analysis
Easy to use equipment with reliable results. Fast analysis and run time.
Review Date: 9 Dec 2016 | Biotage
Clean or dirty samples
Process clean and dirty samples on the same system without changing methods. Biotage® Horizon 3100 handles highly particulated samples with optional Pacific® prefilters, eliminating separate filtration steps. From environmental monitoring to industrial samples, you get consistent recoveries and full compliance with EPA 1664A/B, ISO 11349, and Standard Methods 5520G.
Automated for safety and efficiency
Walk away with confidence. The Horizon 3100 automates every step, from sample loading and bottle rinsing to hexane elution and extract drying. Overflow sensors prevent sample loss, while controlled airflow and the solvent delivery system minimize operator exposure to hexane. Use less solvent, generate less waste, and reduce hands-on time per sample.
Consistent results across your team
Eliminate technician variability with automated bottle rinsing and precise solvent delivery. The intuitive touch-screen controller includes pre-programmed methods for rapid start-up, and three independent stations let you process multiple samples simultaneously. Integrate seamlessly with Speed-Vap® IV for evaporation and optional Solvent Recovery OG for hexane reclamation, creating a complete workflow.
Utilizing 250 mL sample volumes for EPA method 1664B extractions
EPA Method 1664B was developed for measuring oil and grease in wastewater with Hexane Extractable Material (HEM) concentrations ranging from 5 to 1000 mg/L. However, samples containing high oil and grease content (> 500 mg/L HEM) are challenging to extract and therefore the method allows for a smaller sample to be collected. To run reduced sample volumes, laboratories must be able to demonstrate the ability to meet the method detection limit (MDL) requirements at the reduced sample volume level. This application note provides a full solution for EPA method 1664B that meets the method detection limits at 250 mL sample volumes.
Environmental analysis: Smarter sample prep
Environmental analysis of pollutants, water, and soil is essential in preserving and protecting our environment and limiting unnecessary exposure to contaminated material that threatens the livelihood of humans and wildlife. While good water quality is a key contributor to the preservation of a healthy ecosystem, maintaining healthy ecosystems also requires the analysis of soil, waste, and pollution, as well as fats, oils and grease.
In this application guide, learn how solid-phase extraction, developing instrumentation, and media formats can accommodate increasing compound lists, application-specific media, and custom media for specific application challenges. Also, find out how you can solve the challenges laboratories face by creating smarter sample prep workflow solutions that streamline analysis and meet the market requirements and regulatory methods for which they were designed. This eBook covers:
- Drinking water solutions for semi-volatile organic compounds
- Wasterwater: Influent and effluent solutions for semi-volatile organic compounds
- Oil and grease: Avoid setbacks and improve workflows
- Extraction and drying solutions: Hexane extractable material using SPE
- Improving the SPE workflow for 1,4 dioxane in drinking water
- EPH fractionation: Streamline your EPH fractionation process
- Improve throughput and quality in determining diquat paraquat in drinking water
EPA Method 1664B extractions using the Biotage Horizon 3100 with in-line drying using ISOLUTE Sodium Sulfate Drying Cartridges
In this application note, Biotage provides a comphrensive in-line extraction and drying protocol for measuring hexane extractable material (HEM) using solid-phase extraction.
Modern Hexane-Extractable Material (Oil & Grease) Analysis in Wastewater Samples
Oil & grease, is an operationally-defined general measurement used around the world to help assess water pollution due to a variety of hydrocarbons, including dissolved aromatics, benzene, toluene, xylene and dispersed polynuclear aromatic hydrocarbons (PAHs), aliphatics, naphthenic and fatty acids. Some commonly recognized sources of these compounds include fats, greases, soaps, waxes, and oils. In this application note discover how SPE can be used the analyze the presence of oil and grease in wastewater samples.
The Determination of Dispersed Oil in Water Using Automated SPE in Compliance with OSPAR and ISO 9377-2 Methods in Norway
Accurate measurement of dispersed oil-in-water (OIW) has become increasingly more important as international regulations are implemented, requiring off-shore platforms and refineries to demonstrate to regulators and government bodies that effective and accurate OIW monitoring is taking place, as non-conformance of these regulations can result in financial penalties. This application note provides an overview of the OSPAR-recommended method of gas chromatography and flame ionization detection (GC-FID), as described in the modified ISO 9377-2 GC-FID. The use of solid phase extraction (SPE) also eliminates many of the problems that can occur during the extraction step.
Discover the New SPE DEX 3100 Oil and Grease Extractor from Horizon Technology
Watch this video to learn more about automated Oil & Grease analysis from Horizon Technology. See the operation and new WaterTrap™ Drying membrane in action! The SPE DEX 3100 Oil and Grease Extractor handles all types of samples with reliable operation uses the original sample bottle to dispense the sample and automatically rinses it.
Detect Pollution using the New SPE-DEX® 3100 Oil & Grease Extraction System
Hear Zoe Grosser describe the new SPE-DEX® 3100 oil and grease extraction system, which can be used as a general indicatior of pollution. Horizon Technology's new extraction system enables laboratories to detect if harmful compounds are released into the environment. New features include a touch screen, updated software, overflow sensor, safe solvent delivery system and evaporation system. Interview filmed by SelectScience at Pittcon 2015.
How environmental labs can save time and boost productivity with automated solid-phase extraction
Explore the benefits of adopting automated SPE for high-throughput environmental testing
Sample Prep Solved: An Insight into Automated Solid Phase Extraction
Discover how solid phase extraction has improved the quality and throughput of analysis at an environmental lab in Texas

























