µPAC™ GEN2 Limited Sample Amount column
The µPAC™ GEN2 Limited Sample Amount column is designed to maximize protein identifications when working with limited sample amounts.
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The µPAC™ GEN2 Limited Sample Amount column is designed to maximize protein identifications when working with limited sample amounts.
The micro Pillar Array Column technology proves to have exceptional results with regards to reproducibility over time and across laboratories. A second generation design of pillar structure is introduced, where the pillar structure and design have been optimized to enhance the chromatographic separation.
With its 50 cm long and 3 µm deep separation channel allied with a non-porous pillars structure, the column is ideally suited for sample loads up to 20 ng of material where ultra-sensitivity is needed. The flow rate range of this column is 50 to 250 nL/min and the optimal gradient time ranges from 30 min to 120 min.
The µPAC™ GEN2 Limited Sample Amount column is compatible with nanoViper™ fittings and can be integrated on all commercially available nano-LC systems.
Next-generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS analysis in limited sample workflows
In this application note, PharmaFluidics assesses the performance of the µPAC™ GEN2 Limited Sample Amount column against classic packed-bed columns for the bottom-up proteomics when working with low amount of sample on an unmodified nano LC-MS instrument. Download the full note below to explore the potential gain in sensitivity that can be achieved by improving the chromatographic aspect of the limited sample proteomics workflow.
Thermo Fisher Scientific adds patterned microchip-based technology to liquid chromatography portfolio
The technology extends capabilities in column performance for biopharma and proteomics researchers
