LigaFast™ Rapid DNA Ligation System
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…
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Reliable product
Molecular Cloning
Easy to use and high efficiency. Reliable product and does the job.
Review Date: 16 Dec 2021 | Promega Corp.
Time saver!!
Molecular cloning into synthetic bacteria
When time isn't on my side in the lab, this ligase is a life saver. After an easy reaction setup and only a few minutes I have a high proportion of ligated fragments, for which I can transform into my E.coli. Meaning in a day I can have my recombinant plasmids constructed and in my bacteria, opposed to 2 days with other standard ligases.
Review Date: 2 Jul 2019 | Promega Corp.
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).
Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.
LigaFast™ Rapid DNA Ligation System Features & Benefits:
- Flexible - Use with 5´, 3´ or blunt-ended DNA inserts.
- Fast - Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.
- Convenient - No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature.
- Ready-To-Use - No additional buffer modifications required prior to use.
- Efficient - Ligations performed using the LigaFast™ System are comparable to standard overnight ligations.
- Blue/White Cloning Qualified - Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
