LC-4000 Series Autosamplers
The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.
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This product is good for in all aspects. I am totally satisfied to use this in my laboratory.
Review Date: 16 Jan 2015 | JASCO (USA)
The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler.
All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.
Analysis of Protein Hydrolysate Amino Acids using OPA Post-column Derivatization by Quaternary Low Pressure Gradient System
Amino acid analysis has been applied to several categories such as food, medicine, protein science and metabolome study, and is an important measurement technique. An amino acid analysis system using a low pressure gradient unit with OPA post column derivatization provides excellent repeatability and good separation in a short analysis time. The analysis results of food analysis and amino acid composition analysis of protein using this amino acid analysis system are reported.
Analysis of Dabsyl Amino Acids using HPLC
The pre-column derivatization using HPLC for amino acids analysis has prevailed as a simultaneous separation method of multiple components using a selective, high-sensitivity detector and a reversed phase column. Many results of amino acids analysis using dabsyl chloride as a reagent for pre-column derivatization have been reported because the amino acids derivatized by dabsyl chloride are more stable than those by other derivatization agents. Also a simply a UV/VIS detector can be used with high sensitivity. This application note demonstrates the measurement of the standard mixture of amino acids.
Analysis of Amino Acids by Automated Pre-column Derivatization with OPA
Pre-column derivatization methods for amino acid analysis have been widely used in reversed-phase HPLC, as they offer high selectivity and sensitivity for multiple components. Many derivatization reagents are commercially available and one can choose the most suitable reagent for the application. Among them, orthophtalaldehyde (OPA) is one of the most commonly used reagents because it reacts with amino acids very quickly (seconds) at room temperature and derivatized amino acids can be detected by a fluorescence detector offering increased sensitivity. In this application, the OPA pre-column derivatization is demonstrated by using the automated pre-column derivatization function of JASCO’s autosampler for much better reproducibility than manual sampling.
