Analysis of Protein Hydrolysate Amino Acids using OPA Post-column Derivatization by Quaternary Low Pressure Gradient System

Analysis of Protein Hydrolysate Amino Acids using OPA Post-column Derivatization by Quaternary Low Pressure Gradient System

16 Nov 2015

Amino acid analysis has been applied to several categories such as food, medicine, protein science and metabolome study, and is an important measurement technique. An amino acid analysis system using a low pressure gradient unit with OPA post column derivatization provides excellent repeatability and good separation in a short analysis time. The analysis results of food analysis and amino acid composition analysis of protein using this amino acid analysis system are reported.

LC-4000 Series Column Ovens

JASCO (USA)

The LC-4000 Series column ovens have been designed to provide excellent utility for a wide range of different user requirements.For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension). For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or two internally mounted automatic switching valves for 2 or 6 column selection. For GPC and larger preparative columns, select either of the two high-capacity CO- 4060 or CO-4065 ovens. The CO-4060 can accommodate columns with lengths up to 300mm or larger ID preparative columns. The CO-4065 can accommodate up to 10 columns with lengths up to 300mm and a pair of column selection valves is included as standard. All column ovens have built- in electronic cooling for controlling column temperature at sub-ambient temperatures. The CO-4062 and CO-4061 block-heater type ovens can be fitted with an optional micro-volume pre-heating unit for pre- heating the mobile phase before the column to improve resolution and peak shape. The RO-4068 reaction oven is used for high temperature post-column sample processing with capacity for columns and reaction coils.

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LC-4000 Series Autosamplers

JASCO (USA)

The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.

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LC-4000 Series HPLC pumps

JASCO (USA)

The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful solvent delivery system.Accessory modules can be installed in the LC-4000 series pump for use with virtually any solvent delivery requirement. Features Upgrade and Expansion Eliminating pulsation Pump configurations Solvent Mixing Solvent Degassing Preparative and Semi-preparative pumps

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.Amino AcidsMetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Precision MedicinePrecision medicine refers to the idea of customized healthcare, where medical decisions and treatments are tailored to the individual patient. Molecular diagnostics, companion diagnostics and Next Generation Sequencing (NGS) play a pivotal role in this approach. Derivatisation