GoTaq® Probe 1-Step RT-qPCR System
The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimi…
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mRNA detection / quantification
It is critical to have continuous supply during this pandemic. This helped us a lot in addition to the easiness of use and consistent results (due to good product quality controls. I don’t see any issues from one LOT to the other LOT.
Review Date: 16 Jul 2021 | Promega Corp.
The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired.
The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs.
Features - Benefits
• Superior Performance: Sensitive detection on any real-time instrument
• Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection
• Versatility: Compatible with both fast and standard cycling methods
• Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors
• Confidence: The Promega PCR Performance Guarantee
Applications
• Sensitive sequence detection and quantification
• Gene expression analysis
• Detection of sequence variants
• Fluorescent detection
RNA Quantification Using the GoTaq® RT-qPCR Systems
Promega GoTaq® products for RNA quantification offer two dye-based systems for accurate, sensitive, broad-range quantification of RNA targets. This application note demonstrates the successful amplification of 11 differentially expressed mRNA targets using the GoTaq® 1-Step RT-qPCR System. One of these targets was also amplified to demonstrate equivalent performance between the GoTaq® 1-Step and GoTaq® 2-Step RT-qPCR Systems. It was found that reactions with the GoTaq® RT-qPCR Systems have a higher change in fluorescence and earlier Cq values than other RT-qPCR systems.
Optimized Reagents for Probe-Based qPCR using the GoTaq® Probe qPCR and RT-qPCR Systems
The GoTaq® Probe qPCR and RT-qPCR Systems are optimized for qPCR assays using probe-based detection chemistries. This article presents several experiments performed with the GoTaq® Probe qPCR Master Mix that exhibit sensitive quantification and robust performance.
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