FLEXMAP 3D® Multiplexing Instrument

This whitepaper explains how multiplex methods deep bisulfite sequencing and bisulfite PCR-Luminex can be used for DNA methylation analysis in research examining epigenetic modification.

Until recently, none of the commercially available multiplex molecular tests could provide the flexibility to satisfy clinicians' desire for both targeted and broad respiratory pathogen testing in a cost-effective manner for the lab and patient. The VERIEGENE® Respiratory Pathogens Flex Test (RP Flex) is the first commercial test that allows the user to choose any combination of 16 viral and bacterial targets for an individual sample at the time of test ordering, based on the clinician and patient’s needs. The purpose of this white paper is to deliver an overview of the advantages of using VERIEGENE® for your diagnostic needs.

Vaginitis results in >13 million physician office visits per year, and costs over $1 billion to the US healthcare system annually. However, up to 72% of women with vaginitis may remain undiagnosed or misdiagnosed. In this white paper, Luminex presents a summary of the condition and potential ways to effectively diagnose it in patients.

Timely and accurate identification of suspected pathogens in stool specimens from gastroenteritis patients can be challenging due to the similar clinical signs and symptoms in many diarrheal diseases. In this white paper, Luminex describes the use of the xTAG® Gastrointestinal Pathogen Panel (GPP) to accurately identify pathogen species involved in diarrheal and gastrointestinal infections.

Associate Professor Jochen Schwenk discusses how bead-based affinity proteomics, using xMAP technology, is enabling researchers at the KTH Royal Institute of Technology, Stockholm, to map the entire human proteome.

IgA Nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. In this presentation, David Wimbury, a Ph.D. student at University of Leicester, UK, describes a Luminex-based multiplexing immunoassay to investigate the relationship between levels of serum biomarkers associated with B and T cell activation and the development of IgAN. His research highlights the importance of controlling for renal function in serum biomarkers when studying associations between biomarkers and IgAN.

In this presentation, Dr. Stéphane Azoulay, Associate Professor at Université Côte d'Azur, describes the development and application of a multiplex immunoassay to detect biomarkers for use in hazard assessment of aquatic systems.

Anti-cytokine autoantibodies are an emerging field, as their role is increasingly recognized in infectious diseases and inflammation and also as diagnostic tools in various syndromic presentations. In this presentation, Dr. Rainer Döffinger, Cambridge University Hospital, describes the development of a comprehensive panel to screen for serum anti-cytokine autoantibodies using Luminex xMAP technology.

In this presentation, Ramy Huber, MSc., research associate, ProteoMediX AG, describes how Luminex xMAP technology was used to develop a novel immunoassay for biomarkers associated with prostate cancer. Learn how the method has the potential to significantly lower the rate of unnecessary prostate biopsies which are negative for cancer by more than 50%. Learn about current issues surrounding prostate cancer diagnosis, Luminex assay development and clinical testing of the new assay.

A major cause of morbidity and mortality is streptococcus pneumoniae. In this video, Siân Faustini, Ph.D. student at the University of Birmingham, UK, presents her results on assessing immune responses to pneumococcal polysaccharide and conjugate vaccines in HIV. Faustini demonstrates differences in IgG subclass responses to vaccines by using a combination of multiplexed Luminex immunological assays.

The intensive use of antibiotics in animal husbandry and public health care has seen an increase in antimicrobial resistant strains of bacteria. In this video, Jeroen Peters, researcher at RIKILT Wageningen University, Netherlands, presents his findings on the detection of antibiotics using the Luminex multiplex screening technology xMAP®, further demonstrating its validation in raw milk analysis. The presented screening assay could support governmental inspection and may help to further reduce the use of antimicrobials in animal production.

Evelien Germeraad, MSc., researcher at the Wageningen Bioveterinary Research, Wageningen University & Research, Netherlands, describes a powerful method for the fast identification of avian influenza virus subtypes in poultry sera. Hear how the multiplex serological assay based on Luminex xMAP® technology enables the detection of haemagglutinin and neuraminidase surface protein subtypes simultaneously in one assay.

In this presentation, Finn Terje Hegge, chief technical officer of Genetic Analysis (GA), presents its standardized DNA probe-based assay for profiling the gut microbiota. He explains how Luminex xMAP® technology, in combination with the MagPlex® Microspheres, is well suited for the final steps of sorting and detection. Learn how GA is currently developing its clinically validated assay with this Luminex technology.

The excessive production of harmful autoantibodies is a hallmark of autoimmune disease. Recent research has also shown that autoantibody production is useful to characterize cancer patients. The characterization of patient sub groups is essential for the efficient development of therapies for both autoimmune disease and cancer. In this presentation, Hans-Dieter Zucht, Head of Technology, Protagen AG, describes the use of a Luminex-based assay to detect common reactivity patterns of human autoantibodies against endogenous protein targets, using clustered autoantibody reactivities.

We bring you insights, tips and knowledge from 11 experts as they tackle biomarker research using diverse methods