xTAG Gastrointestinal Pathogen Panel for State-of-the-Art Molecular GI Testing

15 May 2018

Timely and accurate identification of suspected pathogens in stool specimens from gastroenteritis patients can be challenging due to the similar clinical signs and symptoms in many diarrheal diseases. In this white paper, Luminex describes the use of the xTAG® Gastrointestinal Pathogen Panel (GPP) to accurately identify pathogen species involved in diarrheal and gastrointestinal infections.

FLEXMAP 3D® Multiplexing Instrument

Luminex

The FLEXMAP 3D® detects up to 500 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents.  Fast read time, compatibility with both 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D the platform of choice for high-throughput bioassay applications. The xMAP® multiplexing platform is composed of an instrument and associated reagents: The FLEXMAP 3D® instrument is based on the principles of flow cytometry, which integrates key xMAP detection components such as lasers, optics, fluidics and high-speed digital signal processors. xMAP microspheres are a family of 500 fluorescently dyed carboxylated polystyrene microspheres that act as both the surface for the solution phase assay and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu commercially available kits. Instrument Details: Multiplex Capacity: 500 Microtiter Plate: 96 well and 384 well Throughput: ~ 20 min/96-well plate (up to 144,000 tests/hour); ~ 75 mins/384-well plate (up to 153,600 tests/hour) Dynamic Range: 4.5 logs Sensitivity:  Detects a minimum of 500 fluorochromes of phycoerythrin (PE) per xMAP microsphere Optics: Lasers/APDs/PMTs Hardware: Flow Cytometry based with dual sample fluidics paths and rapid sampling rate Software: xPONENT® Software offers interfacing options for front-end automation platforms and Laboratory Information Systems (LIS) Dimensions: 23” W x 25.7” D x 18” H (58.4 cm W x 65.3 cm D x 45.7 cm H) Weight: Up to 91 kg (200 lbs), including the instrument, monitor arm, monitor, and accessories, such as the keyboard, mouse and barcode scanner xMAP Reagent Compatibility: Magnetic and nonmagnetic microspheres

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