Akura 384 Spheroid Microplate
The Akura™ 384 Spheroid Microplate is our ultimate spheroid screening plate unifying robust liquid and spheroid handling with automated high-throughput screening applications. The flat bottom and black walls of the InSphero 384-well plate facilitate fluorescent imaging, while the special well design allows medium exchange, dosing, and endpoint determination in the same plate saving time and resources.
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- All-in-one plate allowing spheroid formation, long-term culturing and analysis in one single plate.
- Unique SureXchange™ ledge engineered to prevent unintended spheroid/organoid aspiration and to retain consistent minimal residual volume (2-3 µl) during medium exchange.
- Spheroid/organoid morphology and functionality are preserved in long-term cell culture.
- High-content imaging quality with a continuous, flat-bottom, 125 μm Polystyrene membrane, and a black-walled body.
The package includes 5- or 10-pack of individually wrapped Akura™ 384 Spheroid Microplates, ultra-low-attachment coated, sterile, includes lid.
Semi-automated medium exchange in Akura 96 and 384 Spheroid Microplates with the VIAFLO 96 and VIAFLO 384
This application note describes a semi-automated workflow for medium exchange in 3D spheroid cultures using Akura™ microplates and VIAFLO® multichannel pipetting systems. The approach enables precise liquid handling while preserving spheroid integrity, supporting reproducible and scalable 3D cell culture workflows.
The system combines specialized microplate design with controlled pipetting parameters to minimize spheroid loss and improve experimental consistency. This enables efficient handling of high-throughput assays and long-term cultures, making it well suited to applications in drug discovery, toxicology, and disease modeling.
Resource details:
- Resource type: Application note
- Page count: 7
- Read time: 11 mins
3D aggregation of tumor spheroids in the Akura 384 spheroid microplate
This resource describes the generation of 3D tumor spheroids using the Akura™ 384 Spheroid Microplate, an automation-compatible platform designed for high-throughput in vitro applications. The protocol outlines key steps including plate preparation, cell seeding, and optimization of aggregation conditions to ensure reproducible spheroid formation.
The system enables uniform spheroid formation and supports downstream workflows such as high-content imaging and drug screening. With its scalable 384-well format and compatibility with automated liquid handling, the platform is suited to applications in oncology research, toxicology, and drug discovery.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 8 mins
High-content imaging of tumor spheroids in 3 channels using the Akura 384 Spheroid Plate
This application note describes high-content imaging (HCI) workflows for analyzing 3D tumor spheroids grown in Akura™ 384 microplates. Using confocal imaging and multi-channel fluorescence, the approach enables detailed visualization of cellular structures and spatial organization within spheroids.
The protocol covers sample preparation, staining, clearing, and image acquisition, supporting robust analysis of drug efficacy, toxicity, and cellular interactions. Discover how by combining advanced 3D models with high-resolution imaging techniques, the workflow provides deeper biological insights for drug discovery and preclinical research.
Resource details:
- Resource type: Application note
- Page count: 5
- Read time: 10 mins
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