Akura 96 Spheroid Microplate
The Akura™ 96 Spheroid Microplate is InSphero's most commonly used handling plate for daily spheroid culture in every lab environment, from manual to automated handling. The plate is designed for lossless spheroid formation and handling as well as reliable cell culturing and analysis.
Place your order directly with the manufacturer.
Akura 96 Spheroid Microplates provide a reliable and user-friendly platform for generating uniform 3D spheroids
Toxicology studies
The Akura 96 Spheroid Microplates are easy to use and provide consistent results. Spheroids form reliably within the first 7 days of culture and are highly uniform in size across the plate. The well geometry allows easy handling with a multichannel pipette, making media changes, treatments, and spheroid collection simple and efficient. In addition, the well design enables clear microscopic observation throughout the culture period.
Review Date: 13 Jul 2026 | InSphero
Platform for reproducible spheroid culture and efficient medium exchange compatible with high-throughout handling
Liver safety
The plates provide a robust platform for the aggregation, culture, and long-term maintenance of hepatocyte spheroids. The well geometry, particularly the integrated ledge, facilitates near-complete medium exchange while minimizing spheroid disturbance and tissue loss, thereby improving handling consistency and efficiency of compound exposure/removal. Also, the plate format is compatible with multichannel pipettes and automated liquid handlers, supporting standardized workflows and scalability.
Review Date: 9 Jul 2026 | InSphero
- All-in-one plate allowing spheroid formation, long-term culturing and analysis in one single plate.
- Unique SureXchange™ ledge engineered to prevent unintended spheroid/organoid aspiration and to retain consistent minimal residual volume (5 -7 µl) during medium exchange.
- Spheroid/organoid morphology and functionality are preserved in long-term culture by highly stable, cell-repellent ultra-low attachment coating.
- Enhanced imaging speed and resolution through flat bottom well
- Automation friendly & ANSI/SLAS standard.
- Cyclo-olefin Polymer (COP) plate material for high imaging quality.
The package includes 5 or 20 individually wrapped Akura™ 96 Spheroid Microplates, ultra-low attachment coated, sterile, includes lid.
3D aggregation of tumor spheroids in the Akura 96 Spheroid Microplate
This resource describes the generation of 3D tumor spheroids using the Akura™ 96 Spheroid Microplate, providing a reproducible approach for creating uniform spheroids suitable for in vitro studies. The system’s specialized well geometry supports controlled aggregation, efficient media exchange, and compatibility with imaging and biochemical assays.
The step-by-step workflow covers cell preparation, seeding density optimization, and quality control, enabling consistent spheroid formation across wells. The platform supports long-term culture and high-throughput workflows, making it suitable for applications such as drug screening, tumor modeling, and assay development.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
Semi-automated medium exchange in Akura 96 and 384 Spheroid Microplates with the VIAFLO 96 and VIAFLO 384
This application note describes a semi-automated workflow for medium exchange in 3D spheroid cultures using Akura™ microplates and VIAFLO® multichannel pipetting systems. The approach enables precise liquid handling while preserving spheroid integrity, supporting reproducible and scalable 3D cell culture workflows.
The system combines specialized microplate design with controlled pipetting parameters to minimize spheroid loss and improve experimental consistency. This enables efficient handling of high-throughput assays and long-term cultures, making it well suited to applications in drug discovery, toxicology, and disease modeling.
Resource details:
- Resource type: Application note
- Page count: 7
- Read time: 11 mins
Guide for handling and maintaining microtissues in Akura 96 plates
This resource describes the handling, maintenance, and transfer of 3D InSight™ microtissues in Akura™ 96 Spheroid Microplates. It outlines essential steps including handling upon arrival, medium exchange, and microscopic analysis to ensure reproducible and reliable in vitro results.
The protocol highlights best practices for preserving microtissue integrity, including controlled pipetting techniques and semi-automated workflows. These approaches support consistent long-term culture and downstream applications such as drug testing, imaging, and biochemical assays.
Resource details:
- Resource type: Application note
- Page count: 18
- Read time: 27 mins
3D aggregation of NIH3T3 fibroblasts using the Akura PLUS Hanging Drop System
This resource describes the generation of 3D spheroids using the Akura™ PLUS Hanging Drop System, designed for reproducible and uniform spheroid formation. The workflow covers cell preparation, hanging-drop formation, and optimization of aggregation conditions to ensure consistent spheroid size and composition.
The protocol also details transfer of spheroids to Akura™ 96 microplates for long-term culture and downstream applications. Discover how by combining controlled hanging-drop aggregation with scalable plate-based workflows, the system supports applications in drug discovery, tissue modelling, and in vitro screening.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
Medium exchange with the Akura PLUS Hanging Drop System
This video demonstrates the medium exchange workflow in the Akura™ PLUS Hanging Drop System, a platform designed for the generation and maintenance of uniform 3D spheroids. The process highlights careful liquid handling techniques to preserve drop stability and maintain spheroid integrity during culture.
Discover how the workflow supports consistent spheroid development and seamless transfer to Akura™ 96 microplates for downstream applications. This approach enables reliable long-term culture and integration into drug discovery and in vitro screening workflows.
