Akura PLUS Hanging Drop System
InSphero's world-famous and unique Akura™ PLUS Hanging Drop System in a 96-well format with automation-compatible top-loading. Suitable for many cell types, including cells that do not normally form spheroids or organoids on ULA surfaces.
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The Akura™ PLUS Hanging Drop System is suitable for many cell types, including cells that do not normally form spheroids or organoids on ultra-low attachment (ULA) surfaces.
- Loading of cell suspension from the top through unique SureDrop™ inlet engineered for highly uniform and stable hanging drops.
- Gravity-assisted formation of uniform spheroid/organoid morphology and functionality in long-term culture results in one spheroid per well.
- ANSI/SLAS 96-well standard format for full compatibility with automated liquid handling systems.
- Free-floating spheroids are fully accessible and can be transferred to Akura™ 96 Spheroid Plates for long-term culturing or analysis.
The System contains 10 Akura™ PLUS Hanging Drop Plates and 10 Akura™ 96 Spheroid Plates, individually wrapped, made of polystyrene and cyclo olefin polymer, sterile, includes lid.
3D aggregation of NIH3T3 fibroblasts using the Akura PLUS Hanging Drop System
This resource describes the generation of 3D spheroids using the Akura™ PLUS Hanging Drop System, designed for reproducible and uniform spheroid formation. The workflow covers cell preparation, hanging-drop formation, and optimization of aggregation conditions to ensure consistent spheroid size and composition.
The protocol also details transfer of spheroids to Akura™ 96 microplates for long-term culture and downstream applications. Discover how by combining controlled hanging-drop aggregation with scalable plate-based workflows, the system supports applications in drug discovery, tissue modelling, and in vitro screening.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
The loading step with the Akura PLUS Hanging Drop System
This video demonstrates the loading step with the Akura™ PLUS Hanging Drop System, showing how cell suspensions are dispensed into each well to form stable hanging droplets. Discover how accurate loading supports the generation of spheroids with consistent size and composition, providing a reliable starting point for downstream 3D cell culture experiments and drug discovery workflows.
Medium exchange with the Akura PLUS Hanging Drop System
This video demonstrates the medium exchange workflow in the Akura™ PLUS Hanging Drop System, a platform designed for the generation and maintenance of uniform 3D spheroids. The process highlights careful liquid handling techniques to preserve drop stability and maintain spheroid integrity during culture.
Discover how the workflow supports consistent spheroid development and seamless transfer to Akura™ 96 microplates for downstream applications. This approach enables reliable long-term culture and integration into drug discovery and in vitro screening workflows.
Drop transfer with the Akura PLUS Hanging Drop System
This video demonstrates drop transfer with the Akura™ PLUS Hanging Drop System, where spheroid-containing droplets are transferred from the hanging drop plate into an Akura™ 96 Spheroid Microplate. Explore how the process enables efficient relocation of spheroids for long-term culture and assay preparation.
