Product DemonstrationsDrug Discovery & Development

Medium exchange with the Akura PLUS Hanging Drop System

1 Jul 2026
Medium exchange with the Akura PLUS Hanging Drop System

This video demonstrates the medium exchange workflow in the Akura™ PLUS Hanging Drop System, a platform designed for the generation and maintenance of uniform 3D spheroids. The process highlights careful liquid handling techniques to preserve drop stability and maintain spheroid integrity during culture.

Discover how the workflow supports consistent spheroid development and seamless transfer to Akura™ 96 microplates for downstream applications. This approach enables reliable long-term culture and integration into drug discovery and in vitro screening workflows.

About the company

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InSphero

InSphero sets the standard for in vitro testing of novel drugs in the pharmaceutical and biotechnology industry with comprehensive solutions that provide greater confidence in decision making. Its robust and highly physiologically relevant suite of 3D InSight™ Microtissues and Services are used by major pharmaceutical companies worldwide to increase efficiency in drug discovery and safety testing. InSphero patent-pending technologies and methods enable large-scale, reproducible production of scaffold-free 3D microtissues driven solely by cellular self-assembly. The company specializes in delivering assay-ready and custom 3D models derived from liver, pancreas, and tumor tissues, to provide unrivalled biological insight into liver toxicology, metabolic diseases (e.g., diabetes and liver diseases), and oncology (with a focus on immuno-oncology). All InSphero microtissues are thoroughly validated to ensure the highest quality, certified for use in a variety of assays, and shipped globally to customers in a patented, easy-to-use spheroid-optimized platform, ready for research. Field application scientists and research staff with expertise in working with 3D models help ensure efficient integration and onsite training as needed. For customers who prefer an outsourcing strategy with fast turnaround, InSphero also offers contract research services utilizing their 3D microtissue models.

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Akura™ PLUS Hanging Drop System

InSphero

The world-famous and unique Akura™ PLUS Hanging Drop System in a 96-well format with automation-compatible top-loading. Suitable for many cell types, including cells that do not normally form spheroids or organoids on ULA surfaces.

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Akura™ 96 Spheroid Microplate

InSphero

The Akura™ 96 Spheroid Microplate is our most commonly used handling plate for daily spheroid culture in every lab environment, from manual to automated handling. The plate is designed for lossless spheroid formation and handling as well as reliable cell culturing and analysis.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.3D Cell CultureCulturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular interactions and gene expression. 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies.In VitroIn vitro refers to experiments conducted outside living organisms, often in controlled lab environments such as petri dishes or test tubes. In vitro models are widely used in drug testing, cell biology, and disease research. Explore in vitro research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.