3D aggregation of NIH3T3 fibroblasts using the Akura PLUS Hanging Drop System
30 Jun 2026This resource describes the generation of 3D spheroids using the Akura™ PLUS Hanging Drop System, designed for reproducible and uniform spheroid formation. The workflow covers cell preparation, hanging-drop formation, and optimization of aggregation conditions to ensure consistent spheroid size and composition.
The protocol also details transfer of spheroids to Akura™ 96 microplates for long-term culture and downstream applications. Discover how by combining controlled hanging-drop aggregation with scalable plate-based workflows, the system supports applications in drug discovery, tissue modelling, and in vitro screening.
Resource details:
- Resource type: Application note
- Page count: 4
- Read time: 6 mins
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Akura™ PLUS Hanging Drop System
InSpheroThe world-famous and unique Akura™ PLUS Hanging Drop System in a 96-well format with automation-compatible top-loading. Suitable for many cell types, including cells that do not normally form spheroids or organoids on ULA surfaces.
Akura™ 96 Spheroid Microplate
InSpheroThe Akura™ 96 Spheroid Microplate is our most commonly used handling plate for daily spheroid culture in every lab environment, from manual to automated handling. The plate is designed for lossless spheroid formation and handling as well as reliable cell culturing and analysis.
