Dual binding assay highlights allosteric binding effect on the bispecific Zenocutuzumab
22 Oct 2026This application note explores the use of biolayer interferometry (BLI) to characterize the complex binding behavior of bispecific antibodies. Using Zenocutuzumab as a model, learn how dual binding assays can assess HER2 and HER3 binding individually and sequentially within a single assay. Discover how binding order can influence antigen interactions, how BLI can reveal potential allosteric effects, and how these assays can support the functional characterization and optimization of bispecific therapeutics.
Resource details:
Resource type: Application note
Page count: 10
Read time: 8 mins
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