ResourceDrug Discovery & Development
From DNA template to BLI kinetics in a day
22 Oct 2026Explore a streamlined workflow for producing and characterizing VHH single-domain antibodies using cell-free protein synthesis and biolayer interferometry (BLI). Learn how anti-GFP VHHs produced using NEBExpress® and PURExpress® systems can be quantified and kinetically characterized directly from crude samples using Octet® VHH Biosensors. Discover how combining cell-free expression with label-free BLI analysis can reduce sample preparation and accelerate progression from protein expression to functional binding and affinity measurements.
Resource details:
Resource type: Application note
Page count: 10
Read time: 8 mins
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biophysical Characterization