3D aggregation of tumor spheroids in the Akura 384 spheroid microplate

3 Jul 2026

This resource describes the generation of 3D tumor spheroids using the Akura™ 384 Spheroid Microplate, an automation-compatible platform designed for high-throughput in vitro applications. The protocol outlines key steps including plate preparation, cell seeding, and optimization of aggregation conditions to ensure reproducible spheroid formation.

The system enables uniform spheroid formation and supports downstream workflows such as high-content imaging and drug screening. With its scalable 384-well format and compatibility with automated liquid handling, the platform is suited to applications in oncology research, toxicology, and drug discovery.

Resource details:

  • Resource type: Application note
  • Page count: 4
  • Read time: 8 mins

Akura™ 384 Spheroid Microplate

InSphero

The Akura™ 384 Spheroid Microplate is our ultimate spheroid screening plate unifying robust liquid and spheroid handling with automated high-throughput screening applications. The flat bottom and black walls of the InSphero 384-well plate facilitate fluorescent imaging, while the special well design allows medium exchange, dosing, and endpoint determination in the same plate saving time and resources.

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