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High-content imaging of tumor spheroids in 3 channels using the Akura 384 Spheroid Plate

InSphero
26 Jun 2026

This application note describes high-content imaging (HCI) workflows for analyzing 3D tumor spheroids grown in Akura™ 384 microplates. Using confocal imaging and multi-channel fluorescence, the approach enables detailed visualization of cellular structures and spatial organization within spheroids.

The protocol covers sample preparation, staining, clearing, and image acquisition, supporting robust analysis of drug efficacy, toxicity, and cellular interactions. Discover how by combining advanced 3D models with high-resolution imaging techniques, the workflow provides deeper biological insights for drug discovery and preclinical research.

Resource details:

  • Resource type: Application note
  • Page count: 5
  • Read time: 10 mins

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Akura™ 384 Spheroid Microplate

InSphero

The Akura™ 384 Spheroid Microplate is our ultimate spheroid screening plate unifying robust liquid and spheroid handling with automated high-throughput screening applications. The flat bottom and black walls of the InSphero 384-well plate facilitate fluorescent imaging, while the special well design allows medium exchange, dosing, and endpoint determination in the same plate saving time and resources.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.3D Cell CultureCulturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular interactions and gene expression. 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies.Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.

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