AcroPrep™ Advance Filter Plates for Lysate Clearance
The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols. The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroP…
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The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols.
The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroPrep™ Advance filters plates for lysate clearance provide a prefilter for relatively quick and easy clarification of samples: in addition, the new well geometry ensures faster flow rates and reduced hold up volumes for efficient recovery of biomolecules.
Features:
- Integrated prefilter yields consistent filtration of samples with high levels of gross particulate
- New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume
- Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of the target molecule
Applications:
- Removal of Bacterial debris prior to plasmid purification
- Removal of bacterial and cellular debris prior to protein purification
- Clearance of gross particulates
Efficient Multi-Well Protein Purification Strategies
This application note from Pall Laboratories explains the many different methods used in the protein purification process, as an example the AcroPrep™ Advance 96-well filter plates are used with chromatography media.
AcroPrep™ 96 Filter Plates Reduce the Presence of False Positive Results in Serological Based Immunoassays with Luminex® xMAP® Technology 26/1/16
This poster from Pall Laboratories shows the efficiency of the AcroPrep™ 96 filter plates in assays where false positives can lead to misdiagnosis, with a focus on Thyroid Stimulating Hormone assays which are commonly used in clinical laboratories.
High Throughput Chromatography Sorbent Screening and Purification Process Optimization of a Fab Antibody Fragment Using AcroPrep™ ScreenExpert Plates and PRC Prepacked Columns
Fab antibody fragments are increasingly used for therapeutic applications. Expressed at high yield, they are very specific and lack the Fc portion of the molecule that may lead to undesirable immunological reactions into patients. This study describes the development of a three-step purification process for a fragment of antibody (Fab) expressed in Pichia pastoris.
Lysate Clearance Using the AcroPrep™ Advance Filter Plate
Molecular biology methods have led to the successful production of a variety of synthesized biomolecules (DNA, RNA, proteins) from prokaryotic and eukaryotic cell lines. The most problematic step in the purification of these biomolecules is the clarification of the sample once the cells are lysed. This application note presents a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples.
Solution for Effective Removal of Cell Debris and Subsequent Purification of Biomolecules
This technical note by Pall Life Sciences presents a solution for the most problematic step in the purification of biomolecules which is the clarification of the sample once the cells have been lysed. In the past, centrifugation was the primary method used to sediment cellular debris, this method has several limitations. However, Pall Life Sciences present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples. The filter plate provides maximum biomolecule recovery and effectively removes cellular debris from samples prior to purification.
Innovations in Sample Clean Up and Recovery for Molecular Biology
Discover the innovative solutions from Pall Life Sciences, designed to enhance your sample recovery and purification for molecular biology applications. Product Manager, Kris Bolen, explains how Pall's centrifugal devices and filter plates can optimize sample recovery and clean up for a range of downstream analyses and processes.
New Products for High Performance Sample Prep
Watch this interview with Pall Life Sciences to learn about the Microsep™ Advance Centrifugal Devices, for ultrafiltration and microfiltration, and AcroPrep™ Advance Filter Plates. The optimised format and membrane configuration of these filter plates improves bead recovery and sampling in multiplexing assays. Pittcon 2011 interview with SelectScience.tv.
