SelectScience InterviewsLife Sciences

Innovations in Sample Clean Up and Recovery for Molecular Biology

16 Feb 2016

Innovations in Sample Clean Up and Recovery for Molecular Biology

Discover the innovative solutions from Pall Life Sciences, designed to enhance your sample recovery and purification for molecular biology applications. Product Manager, Kris Bolen, explains how Pall's centrifugal devices and filter plates can optimize sample recovery and clean up for a range of downstream analyses and processes.

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Established in July 1998, SelectScience is the fastest way to impartial, expert opinion about the best laboratory equipment and latest techniques.

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AcroPrep™ Advance 96-Well Filter Plates for DNA Purification
Pall Life Sciences - Laboratory, Food, Beverage
Maximum Yields of High Quality DNA. Optimized for maximum binding yield of DNA from a variety of sample types New well geometry results in reduced hold-up volume and higher recovery of DNA New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment
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AcroPrep™ Advance 96-Well Filter Plates for Multiplexing
Pall Life Sciences - Laboratory, Food, Beverage
Superior Bead Recovery and Low Levels of False Positives Ensure Assay Reproducibility. Smooth well wall provides efficient bead recovery, ensuring reproducible results lot after lot High performance membrane does not trap microspheres in the membrane matrix In serological assays, Supor® membrane effectively removes IgG complexes, thus reducing nonspecific reactivity of the microspheres and reducing false positives New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume New outlet tip geometry minimizes sample leakage and loss during incubation steps so that acquisition times are not affected Intrinsic plate and membrane properties minimize target loss from non-specific binding
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AcroPrep™ Advance 96-Well Filter Plates for Solvent Filtration
Pall Life Sciences - Laboratory, Food, Beverage
Robust Chemical Resistance Provides Compatibility with Harsh Organics. Chemically resistant materials provide a stable platform to process samples in organic solvents Inert materials of construction ensure complete recovery of samples and low non-specific binding New well design results in faster, more uniform filtration rates across the plate and reduces hold-up volume Innovative outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross-contamination
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AcroPrep™ Advance Filter Plates for Lysate Clearance
Pall Life Sciences - Laboratory, Food, Beverage
The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols. The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroPrep™ Advance filters plates for lysate clearance provide a prefilter for relatively quick and easy clarification of samples: in addition, the new well geometry ensures faster flow rates and reduced hold up volumes for efficient recovery of biomolecules.Features: Integrated prefilter yields consistent filtration of samples with high levels of gross particulate New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of the target molecule Applications: Removal of Bacterial debris prior to plasmid purification Removal of bacterial and cellular debris prior to protein purification Clearance of gross particulates
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Nanosep® & Nanosep MF Centrifugal Devices
Pall Life Sciences - Laboratory, Food, Beverage
Simple, reliable concentrating and desalting of 50 to 500 µL samples. Ensures rapid processing of samples. Typical recoveries are greater than 90%. Available with low protein binding Omega™, Bio-Inert®, and GHP membranes. A wide range of MWCOs, color-coded for easy identification. Constructed of low-binding polypropylene. Ultrasonically welded seals prevent bypass or seal failure. Fits standard centrifuge rotors that accept 1.5 mL tubes.
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Innovations in Sample Clean Up and Recovery for Molecular Biology