Nanosep® & Nanosep MF Centrifugal Devices
Simple, reliable concentrating and desalting of 50 to 500 µL samples. Ensures rapid processing of samples. Typical recoveries are greater than 90%. Available with low protein binding Omega™, Bio-Inert®, and GHP membranes. A wide range of MWCOs, color-coded for easy identification. Constructed of low-binding polypropylene. Ultrasonically welded seals prevent bypass or seal failure. Fits standard centrifuge rotors…
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- Simple, reliable concentrating and desalting of 50 to 500 µL samples.
- Ensures rapid processing of samples.
- Typical recoveries are greater than 90%. Available with low protein binding Omega™, Bio-Inert®, and GHP membranes.
- A wide range of MWCOs, color-coded for easy identification.
- Constructed of low-binding polypropylene.
- Ultrasonically welded seals prevent bypass or seal failure.
- Fits standard centrifuge rotors that accept 1.5 mL tubes.
Nanosep Centrifugal Ultrafiltration Devices: Uses for Cloning and PCR
Molecular cloning is a technique often used in most areas of biological research. Standard molecular cloning techniques require enzymatic modification and purification steps to prepare DNA sequences for litigation and transformation into bacterial cells. The polymerase chain reaction (PCR) is employed to generate more of the desired DNA fragment ready for cloning. The use of Nanaosep® centrifugal ultrafiltration devices in various steps of the cloning process can provide a more streamlined process compared to more traditional methods. This application note demonstrates how DNA products from PCR were concentrated and desalted while simultaneously allowing primers and adapter fragments to pass into the discarded filtrate, saving time and materials while providing results similar to those obtained via traditional methods.
Innovations in Sample Clean Up and Recovery for Molecular Biology
Discover the innovative solutions from Pall Life Sciences, designed to enhance your sample recovery and purification for molecular biology applications. Product Manager, Kris Bolen, explains how Pall's centrifugal devices and filter plates can optimize sample recovery and clean up for a range of downstream analyses and processes.
Pall Presents Filtration and Sample Prep Technologies for Lab Automation
Learn about the range of Pall Life Sciences products for purification and sample preparation. The unique AcroPrep™ Advance96- and 384-well filter plates are ideal for applications including lysate clearance, DNA binding, multiplexing, and protein purification. Advance Centrifugal Devices enable sample volumes ranging from 500 µL to 60mL, and Acrodisc® Syringe Filters can be used for HPLC, UHPLC and dissolution testing.
Optimizing Protein Purification Workflows at Cayman Chemical
Dr Tony Ludlam, Protein Core Manager, Cayman Chemical, tells SelectScience® about the technology improving protein production
