ACQUITY UPLC® BEH C18 and C8 Columns
ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these…

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Perfect column for metabolomic purpose!
Untargeted metabolomic profiling of Human Blood/Plasma
UPLC BEH C18 Premier column is very efficient in terms of reproducibility and sensitivity. Peak shapes in the column are very sharp and minimum tailing was observed.
Review Date: 14 Apr 2022 | Waters
The best.
Analysis of toxins
Really effective column. Its life is very long, particularly if used with its column guard.
Review Date: 27 Sept 2021 | Waters
Durable, excellent separation and reproducible results.
Analysis of plant secondary metabolites
Durable HPLC column capable of delivering excellent separation of flavonoid compounds with reproducible results.
Review Date: 26 Jul 2021 | Waters
Great performance.
Separation
Great performance & separation.
Review Date: 21 Jun 2021 | Waters
Good efficiency, very robust column.
Analyze azo dyes in textile samples
We currently use BEH C18 1.7 um 2,1x100mm columns to analyze textile samples searching for azo dyes. We assess textile samples to know if they comply with current European in force legislation. These columns are very robust and we get very reproducible results week by week. We analyze near 100 samples in a week. It is very satisfying to work with such products.
Review Date: 7 Jun 2021 | Waters
Waters' Acquity UPLC BEH C18 and C8 columns: A great chromatography solution.
Aanalyze small molecules
Waters' Acquity UPLC BEH C18 and C8 columns have the highest reliability for retaining molecules with sustained repeatability. Good chromatography with long life for analysis of small molecules.
Review Date: 17 Sept 2020 | Waters
A go to column.
environmental analysis.
Very robust column that serves well over time. Especially if you take care of it (a good cleaning especially after highly concentrated samples). A very much go to column, one of the most versatile columns I've used for various pharmaceutical analyses in an aquatic (environmental) analysis.
Review Date: 21 Nov 2019 | Waters
Best UHPLC column ever.
Chromatographic separation of persistant organic pollutants
Some colleagues suggested this column to me because of its easy set-up and Amazing peak shape and resolution. I used it for just a few months and I can confirm their opinion. This is the best column that I ever used. It is a little expensive but its performance is priceless.
Review Date: 18 Dec 2018 | Waters
Reliable and long lasting.
Purity check
Very reliable columns with good reproducibility expected from the brand.
Review Date: 8 Mar 2017 | Waters
BEH columns are great for analytical analysis.
Analytical chemistry
Waters BEH columns are very robust and easy to use. I run ~2000 samples on the same column, and the performances of the column are very reproducible. I usually run in-house standard mix to evaluate the performance of the column and the UPLC system to assure the experimental results. In all, the BEH column can give very reproducible results with retention time shift within 0.02min, which is very important for any analysis.
Review Date: 11 Jan 2017 | Waters
ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.
They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these new chemistries also utilize new, proprietary endcapping processes which produce outstanding peak shape for bases. These bonding chemistries and particle synthesis innovations produce the sharpest peaks, highest efficiencies and maximum MS sensitivities.
Brochures
Acquity UPLC Columns Brochure
When investing in a new LC instrument, it is important to consider its capabilities to meet existingand future demand. With UPLC Technology, one can invest in the future by utilizing a single system with the capabaility to optimally run sub-2μm UPLC Columns with the ruggedness and robustness to run legacy HPLC methods. The ACQUITY UPLC® System family is an LC Technology platform that can be utilized independent of your separation needs, thus facilitating productivity and simplifying method transfer from site to site.
Streamlined Multivariate Analytical Tools to Determine Compositional Aqueous Film-Forming Foams (AFFFs) Subject to Environmental Release
Used to extinguish flammable liquid fuels, AFFFs consist of numerous fluorocarbon and hydrocarbon compounds. Tracking these constituents' inadvertent release into the environment is important. This application note demonstrates the use of UNIFI software for easy identification of the markers of interest using a multivariate analysis approach.
Acquity UPLC Analysis of Edible Oils
This application note describes a 10-minute separation and identification method for analysis of edible oils with Waters® ACQUITY UPLC®/PDA system using low toxic solvents, acetonitrile and 2-propanol as the mobile phase. The UPLC/PDA method not only allows faster and more precise analysis of triglycerides to authenticate the edible oils but also detects oxidized and decomposed triglyceride, enabling simultaneous determination of oil purity.
Acquity UPLC Analysis of Olive Oil Quality & Adulteration
This application note describes a 10-minute method for olive oil analysis with Waters® ACQUITY UPLC®/PDA system using low toxicity solvents, acetonitrile and 2-propanol as the mobile phase. The UPLC/PDA method allows fast and precise analysis of triglycerides to authenticate olive oil and can be used to unambiguously identify the adulteration of olive oil.
Ultrapure Water for UHPLC
This application note compares the accuracy of results when using LC-MS grade, ULC/MS grade, and ultrapure water to determine a variety of analytes.
High Throughput Regulatory Analysis of Phthalates and Parabens in Cosmetics and Personal Care Products, using UPLC with Mass Dectection
Phthalates and parabens are extensively used as preservatives and stabilizing agents in cosmetics and personal care products. Many are considered as hazardous, affecting the reproductive system and possibly carcinogenic. Many manufacturers are developing and labeling cosmetic and personal care products ‘free-from’ phthalates and parabens. This poster considers the method development, sample extraction and mass spectral analysis of parabens, phthalates, and triclocarben using UPLC coupled to a novel mass detector.
An Improved QuEChERS Method for UPLC-MS/MS Determination of Multiresidue Mycotoxins in Grains
Mycotoxins are toxic compounds produced by molds or other fungi that can grow on foodstuffs intended for domestic animal or human consumption. Ingestion of food containing part-per-billion concentrations of some mycotoxins may cause severe illness. In this poster, an evaluation of the QuEChERS method is presented for the determination of mycotoxins in whole grain wheat, maize and rice flours.
Targeted and Untargeted Lipidomics Using an Integrated Microfluidics Mass Spectrometry Technology
This poster describes a fast and robust microfluidics platform for the analysis of lipidomics that increased the sensitivity whilst considerably reducing the solvent consumption. This novel platform integrates the UPLC separation into the source of the mass spectrometer.
Transferring an Analytical-Scale LC Method to an Integrated Microfluidic Device for Quantification of Human Insulin and 5 Analogs in Plasma
This application note investigates how ionKey technology led to improved sensitivity for insulin and it's analogues, achieving levels of quantification of 25pg/ml whilst reducing plasma sample and injection volumes. This method was carefully optimized to maximize sensitivity using a multi-dimensional LC system.
A Rapid and Sensitive LC-MS/MS Method for the Analysis of Three Forms of Thyroid Hormones Using Raptor™ Biphenyl LC Columns
This application note demonstrates an LC-MS/MS method for the analysis of thyroid hormones at the free form levels using the highly efficient and selective Raptor™ Biphenyl LC column. Accurate and sensitive measurement of low pg/mL levels of free hormones is necessary to assess thyroid function for both veterinary and human diagnostics.
Exploring the Benefits and Potential of iKey Microfluidic Chromatography and Time-of-Flight Mass Spectrometry for Pesticide Residue Analysis
This study aims to characterize sensitivity enhancements and reduction of matrix suppression for residue analysis. Learn how IonKey/MS offers some unique advantages for profiling complex matrices.
Improved LC-MS analysis of oligonucleotides with next-generation chromatography
Join us Wednesday, November 18, to discover the impact metal interactions have on oligo analysis
Waters introduces Atlantis PREMIER Columns to accelerate pharmaceutical methods development
The first of Waters' new PREMIER class of liquid chromatography products
Webinar Highlights: Developing Analytical Chromatographic Methods for Pharmaceutical Storage Stability Investigations
Chromatographic method development factors, parameters and strategies
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