Acquity UPLC Columns Brochure

21 Mar 2017

When investing in a new LC instrument, it is important to consider its capabilities to meet existingand future demand. With UPLC Technology, one can invest in the future by utilizing a single system with the capabaility to optimally run sub-2μm UPLC Columns with the ruggedness and robustness to run legacy HPLC methods. The ACQUITY UPLC® System family is an LC Technology platform that can be utilized independent of your separation needs, thus facilitating productivity and simplifying method transfer from site to site.

Related products

Request Quote for All Products

ACQUITY UPLC BEH Columns
Waters
In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid (BEH) particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer: Excellent peak shape and efficiency for basic analytes A rational array of chromatographic selectivity Improvements in chemical stability at mobile phase extremes, particularly at elevated pH
(41)
ACQUITY UPLC® BEH C18 and C8 Columns
Waters
ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these new chemistries also utilize new, proprietary endcapping processes which produce outstanding peak shape for bases. These bonding chemistries and particle synthesis innovations produce the sharpest peaks, highest efficiencies and maximum MS sensitivities.
(15)
ACQUITY UPLC® BEH Shield RP18 Columns
Waters
ACQUITY UPLC® BEH Shield RP18 columns combine Waters patented Shield Technology* with BEH Technology™ by incorporating an embedded carbamate group into the bonded phase ligand.Waters shield ligands consistently demonstrate increased retention of phenolic compounds versus straight chain alkyl columns. The alternate selectivity and excellent peak shape from the embedded polar group ligand, when combined with the wide pH range and ultra-efficiency of the 1.7 μm BEH particle, provide a necessary and powerful tool for UPLC® method development.
(3)
ACQUITY UPLC® BEH Phenyl Columns
Waters
ACQUITY UPLC® BEH Phenyl columns utilize a trifunctional C6 alkyl tether between the phenyl ring and the silyl functionality. This ligand, combined with the same proprietary endcapping processes as the ACQUITY UPLC™ BEH C18 and C8 columns, provides ultra-low column bleed, long column lifetimes and excellent peak shape. This unique combination of ligand and endcap on the 1.7 μm BEH particle creates a new dimension in selectivity and efficiency for challenging UPLC® separations.
(0)
ACQUITY UPLC CSH and XSelect Columns
Waters
Introducing Waters ACQUITY CSH and XSelect Columns - Powered by CSH Technology Waters patent-pending, Charged Surface Hybrid (CSH™) Technology powers the new Waters ACQUITY CSH and XSelect Column families.CSH Technology columns offer separation scientists a wider range of selectivities and greater performance in LC/MS-compatible mobile phases. Waters CSH Technology empowers UPLCand HPLC systems to provide separations with:Widest selectivity rangeSharper peak shapes for ionized compoundsSeamless transferabilityHigher peak capacitiesRapid equilibration after mobile-phase pH changes
(8)
ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
Waters
ACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns. Easier Method Transfer Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time. Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.
(13)
ACQUITY UPLC® BEH HILIC Columns
Waters
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in HILIC are prone to dissolution at neutral pH. The rugged BEH particle’s wide usable pH range overcomes this pH limitation and results in long column lifetimes. ACQUITY UPLC™ BEH HILIC columns offer advantages such as retention and separation of very polar basic compounds, alternate selectivity as compared to reversed-phase chromatography, increased ESI-MS sensitivity, easier sample preparation, high efficiency and long column lifetimes.
(5)
XBridge® and ACQUITY UPLC® Oligonucleotide C18 Columns
Waters
XBridge® and ACQUITY UPLC® Oligonucleotide C18 Columns for Synthetic Oligonucleotide Analysis and PurificationIon-pair, reversed-phase separation of detritylated oligonucleotides on sub-3 micron particles yields exceptional component resolution.UPLC ® technology increases sample throughput while maintaining necessary oligonucleotide component resolution.LC/UV and LC/MS methods for enhanced quantitative and qualitative oligonucleotides analyses.Various XBridge™ Oligonucleotide C 18 column dimensions address lab scale purification needs.2nd generation hybrid-silica BEH Technology™ particles deliver exceptional column life.Use of synthetic oligonucleotides for research, diagnostic, and therapeutic applications continues to increase. The availability of 2.5 μm HPLC or 1.7 μm UPLC ® technology particles in several column dimensions provides flexibility to meet various LC/UV or LC/MS application needs. Waters' stringent manufacturing and quality control procedures help ensure consistent batch-to-batch and column-to-column performance.
(3)