ACQUITY UPLC® BEH HILIC Columns
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Great product.
Various HILIC applications for LC-MS of polar analytes
Very efficient separations, we have used the columns for more than 12 years. We practically base more than 12 protocols on sugars, aminoacids, amines, nucleotides analysis. Reproducible and long life.
Review Date: 30 Oct 2020 | Waters
The column is good for small molecule separation.
Separation of compounds so that they can be detected later in detector
This column is easy to maintain. The perfomance of this column is suitable for the separation of small polar molecules. We used this column to do metabolomic analysis.
Review Date: 26 Mar 2019 | Waters
LC-MS/MS endocrinology
It is not very easy to work with HILIC columns, but it does works. If I had to choose now, maybe I would prefer to do reverse phase chromatography for this application. The HILIC amides are the more robust columns.
Review Date: 27 Jan 2014 | Waters
QC Analysis
Columns have low use value, they don't tend to last long. However, a short run time is a plus and it saves time on long analyses.
Review Date: 22 Mar 2012 | Waters
I like to use this column for my work. It works well and the only obstacle is having to prepare salt solution although this is not too much work.
Review Date: 13 Apr 2011 | Waters
ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.
These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in HILIC are prone to dissolution at neutral pH. The rugged BEH particle’s wide usable pH range overcomes this pH limitation and results in long column lifetimes. ACQUITY UPLC™ BEH HILIC columns offer advantages such as retention and separation of very polar basic compounds, alternate selectivity as compared to reversed-phase chromatography, increased ESI-MS sensitivity, easier sample preparation, high efficiency and long column lifetimes.
Brochures
Acquity UPLC Columns Brochure
When investing in a new LC instrument, it is important to consider its capabilities to meet existingand future demand. With UPLC Technology, one can invest in the future by utilizing a single system with the capabaility to optimally run sub-2μm UPLC Columns with the ruggedness and robustness to run legacy HPLC methods. The ACQUITY UPLC® System family is an LC Technology platform that can be utilized independent of your separation needs, thus facilitating productivity and simplifying method transfer from site to site.
A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-Line Enrichment and Class Specific Separation of Phospholipids
Lipidomics is a blossoming field of study for identifying biomarkers and potential drug targets in cancer research. In this application note, learn how Waters UPLC and tandem quadrupole MS technologies deliver a highly productive workflow from extraction to separation, identification and quantification of phospholipids
Metabonomics: Understanding Human Metabolism
Dr Matthew Lewis describes how the MRC-NIHR Phenome Centre investigates what makes people metabolically different. By analyzing large cohorts of patients and performing epidemiological studies, an insight into the understanding of human metabolism can be achieved. NMR and UPLC mass spectrometry are used with a combination of different chromatography methods to gain accurate analysis of small molecules in human samples.
Separating polar analyte mixtures: Benefits of a new zwitterionic HILIC chemistry
Watch this on-demand webinar to discover a new zwitterionic HILIC stationary phase that addresses the need for improved pH stability
Webinar Highlights: Developing Analytical Chromatographic Methods for Pharmaceutical Storage Stability Investigations
Chromatographic method development factors, parameters and strategies
Waters New HILIC-based Glycoprotein Column Enhances Glycan Analysis
Provides glycan occupancy information for biotherapeutic glycoproteins
