ACQUITY UPLC CSH and XSelect Columns

This application note describes a simple, rapid, and routine LC-MS method that was developed for the characterization and analysis of lipid nanoparticle (LNP) components using an ACQUITY Premier CSH C18 Column and the BioAccord System.

Previous studies involving the excision of heart tissue from zebrafish have shown tissue to regenerate in only a few days following amputation. Detailed analysis using genomic and proteomic approaches have identified a number of potential candidates that may be implicated in the regeneration process.

This application note uses a multi-omics approach to study zebrafish plasma, rather than the excised tissue, to provide a greater understanding of the mechanisms involved in heart tissue regeneration following the operation. Lipidomic and proteomic data have been collected using a label-free LC-HDMS approach.

The ability to analyze peptides and proteins is becoming increasingly important in the field of biotheraputics. This infographic explains why quantifying large molecules is challenging and how Waters can provide solutions.

Often in protein based biotherapeutics development peptides are analyzed to assess the quality of candidate molecules using optical based assays. While this provides acceptable results orthogonal detection techniques, such as mass detection, can improve specificity, expand detection limits and increase confidence about peak homogeneity. In this application note Waters demonstrate the ACQUITY QDa Detector for the acquisition of optical and MS-based data.

This application note demonstrates how increased confidence in the identification of extractable or leachable compounds can be provided by the inclusion of collisional cross section (CCS) values, acquired using ion mobility-mass spectrometry. The CCS depends on an ion's size, shape and charge, and is a key physicochemical property of compounds.

This work demonstrates that the ACQUITY QDa Detector is compatible with conventional LC mobile phases and is able to detect and accurately report mass information for peptides over a wide molecular weight range typical of biotherapeutic peptide maps. These results establish the ACQUITY QDa Detector as an ideal addition for the biopharmaceutical analyst’s lab that will afford increases in productivity and the confidence of data analysis for routine peptide analysis assays.

In this application note, a comparison is performed between Ostro Pass-through Sample Preparation and SSLE. Typical samples used in drug discovery and development with different acidic, basic and neutral properties and hydrophobicities were analyzed in this evaluation. Key areas of comparison are procedure simplicity, analyte recoveries, matrix factor, and batch-to-batch reproducibility.

This application note describes a simple, 3-step SPE protocol using OasisHLB in µElution Plates and 1 cc cartridges. This protocol saves an average 40% processing time, 70% solvent consumption, and provides a simple method that works for a wide range of acidic, neutral, and basic analytes. This simplified, 3-step protocol consists of load, wash, and elution, thus eliminating the conditioning and equilibration steps.

In this application note, milk and ground beef are prepared and analyzed for avermectins at the ppb level using QuEChERS methodology and LC-MS/MS.