ResourceSeparations

A Simplified Solid Phase Extraction (SPE) Protocol for Bioanalysis Using Oasis HLB

6 Oct 2014

This application note describes a simple, 3-step SPE protocol using OasisHLB in µElution Plates and 1 cc cartridges. This protocol saves an average 40% processing time, 70% solvent consumption, and provides a simple method that works for a wide range of acidic, neutral, and basic analytes. This simplified, 3-step protocol consists of load, wash, and elution, thus eliminating the conditioning and equilibration steps.

Oasis® µElution Plate

Waters

The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.

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ACQUITY UPLC CSH and XSelect Columns

Waters

Introducing Waters ACQUITY CSH and XSelect Columns - Powered by CSH Technology Waters patent-pending, Charged Surface Hybrid (CSH™) Technology powers the new Waters ACQUITY CSH and XSelect Column families.CSH Technology columns offer separation scientists a wider range of selectivities and greater performance in LC/MS-compatible mobile phases. Waters CSH Technology empowers UPLCand HPLC systems to provide separations with:Widest selectivity rangeSharper peak shapes for ionized compoundsSeamless transferabilityHigher peak capacitiesRapid equilibration after mobile-phase pH changes

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A Simplified Solid Phase Extraction (SPE) Protocol for Bioanalysis Using Oasis HLB