ACQUITY UPLC M-Class System

Since its introduction in 2004, each addition to the Acquity family has continuously pushed forward to enable you to find faster, simpler and better ways to support the work that you do.

RAMMP studies have shown comparable group discrimination and improved selectivity over conventional UPLC chromatography. This application note demonstrates further improvements to the workflow by coupling RAMMP with a novel DIA method (SONAR), providing highly specific and unbiased two-dimensional metabolomic data.

SONAR is an acquisition technique comprised of a low-resolution quadrupole mass filter, which is scanned repetitively and both precursor and MS-MS data are acquired at spectral rates approaching 2000 spectra/s.

Previous studies involving the excision of heart tissue from zebrafish have shown tissue to regenerate in only a few days following amputation. Detailed analysis using genomic and proteomic approaches have identified a number of potential candidates that may be implicated in the regeneration process.

This application note uses a multi-omics approach to study zebrafish plasma, rather than the excised tissue, to provide a greater understanding of the mechanisms involved in heart tissue regeneration following the operation. Lipidomic and proteomic data have been collected using a label-free LC-HDMS approach.

SONAR™ is the newest data independent aquisition (DIA) mode from Waters and is complementary to MS. The strength of SONAR is its increased selectivity, which translates to cleaner MS/MS data, allowing both discovery and quantification from a single injection using commonly accepted and established workflows.

SONAR delivers this increased selectivity with rapid scan rates to provide the following analytical benefits:

• All the information required for quantification and identification in a single injection
• Scan rates compatible with UPLC speeds for reliable quantitation
• Library searches are more reliable due to cleaner MS/MS spectra
• Laboratory workflows become more efficient as time consuming method development associated with MS/MS analysis is removed

This white paper discusses in detail the principles and applications of SONAR

This white paper reviews various experimental parameters that influence the chromatographic performance of a trap-and-elute nanoLC method. The most important step in the method development process is to select an appropriate column and trap pair that maximizes chromatographic resolution so that the method is capable of thoroughly identifying proteolytic peptides.

Egg based proteins are one of the most frequent causes of adverse reactions in food. Since many processed foods contain egg as a raw ingredient, the ability to assess changes in protein structure and detection through the manufacturing cycle is important. Although only egg proteins were the focus of this work, other proteins relating to other allergens were observed, providing a potential means for multi-allergen detection.

Ginseng is a slow-growing perennial plant believe to offer different therapeutic benefits such as CNS stimulant activity, hypoglycemic properties and sedative effects. Ion mobility is a rapid orthogonal gas separation phase technique that allows another dimension of separation to be obtained within an LC timeframe. This application note investigates the use of IM separation in combination with microflow chromatography to provide a route to specific and unambiguous identification of ginsenosides.

Stevia rebaudiana Bertoni is a perennial shrub of the Asteraceae (Compositae) family which is of great economic value due to the high content of natural, dietetically valuable sweeteners in its leaves. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) has established regulations for steviol glycosides demanding a purity level of at least 95% of the seven chemically defined steviol glycosides. Complete residue analysis of steviol glycosides in food, is a challenge due to nature of the task i.e. the detection of low steviol glycoside isomer concentrations in complex food commodities, where generic extraction procedures have been used. This application note illustrates the selectivity of collision cross section (CCS) measurements used in combination with other recent MS technology enhancements for profiling complex mixtures.

This study combines μElution SPE and the novel and highly efficient ionKey/MS system to improve a quantitative assay for teriparatide in human plasma. Teriparatide is a recombinant form of a fragment of human parathyroid hormone, used in the treatment of osteoporosis. Peptides in general are often difficult to analyze by LC-MS/MS, as MS sensitivity is low due to the formation of multiple precursors and poor or overly extensive fragmentation. IonKey/MS can provide increased sensitivity compared to 2.1 mm I.D. chromatography with the same injection volume, or equivalent or greater sensitivity with reduced sample consumption, making it ideal for peptide analyses.

In this application note, we illustrate the selectivity of collision cross section (CCS) measurements used in combination with other recent MS technology enhancements for profiling complex mixtures. A combination of high resolution mass spectrometry and high efficiency ion mobility based measurements and separations is used. Ion mobility is a rapid orthogonal gas separation phase technique that allows another dimension of separation to be obtained within an LC timeframe. Compounds can be differentiated based on size, shape, and charge.

How the Acquity UPLC system can save you time, money and solvent consumption