Considerations for Selecting the Optimal Stationary Phases for Proteomic Trap-and-Elute Nanochromatography

This white paper reviews various experimental parameters that influence the chromatographic performance of a trap-and-elute nanoLC method. The most important step in the method development process is to select an appropriate column and trap pair that maximizes chromatographic resolution so that the method is capable of thoroughly identifying proteolytic peptides.