High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Classic reversed-phases for all general-purpose applications. Features: Excellent peak shape and efficiency compared to competitive columns Classic reversed-phase retention and selectivity Excellent, no-bleed character for LC-MS applications C18 is generally more retentive than the C8 Column Details: Chemistry: C18 Particle Size: 5 µm Pore Size: 180 Å Length: 20, 30, 50, 100, 125, 150, 250 mm ID: 2.1, 3.0, 4.…
ACQUITY UPC2 Column Technology for Chiral and Achiral Separations. ACQUITY UPC2 Trefoil™ 2.5 µm Columns are uniquely designed for the ACQUITY UPC2 System. Selectivity and speed for chiral separations Reduce method development time Based on modified polysaccharide-based stationary phases for broad-spectrum chiral selectivity. ACQUITY UPC2 Torus™ Columns simplify the method development process with four completely new…
Empower™ 3 Method Validation Manager (MVM), an option for Empower 3 Chromatography Software, allows you to perform chromatographic method validation, from protocol planning through final reporting, in one application.With Empower 3 MVM you can automatically: Manage method validation workflow in one comprehensive, automated application Clearly display the status of ongoing validation studies – enabling you to see at what…
The use of an ultra pure, ultra inert silica has many recognised benefits, including improved reproducibility, lifetime and chromatographic performance (particularly with basic molecules). However, since the ultra inert silica surface effectively no longer contributes to the separation, C18 columns manufactured with high purity silicas show near identical selectivity. It is therefore highly likely that a problem separation on…
Full Range SelectivityFour unique phases developed to provide a different selectivity for successful separations of the most complex mixtures and challenging analytes under reversed phase conditions. Whether your compounds are hydrphobic or polar, Synergi columns provide you with a full range of selectivity, ensuring separation of the most challenging and complex mixtures.
The LC-4000 series HPLC pumps include expansion bays that allow a simple pump module to be transformed into a powerful solvent delivery system.Accessory modules can be installed in the LC-4000 series pump for use with virtually any solvent delivery requirement. Features Upgrade and Expansion Eliminating pulsation Pump configurations Solvent Mixing Solvent Degassing Preparative and Semi-preparative pumps
The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.
The LC-4000 Series column ovens have been designed to provide excellent utility for a wide range of different user requirements.For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension). For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or two internally mounted automatic switching valves for 2 or 6 co…
The Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. This ultra high performance liquid chromatography system shares all Vanquish values, such as a design focused on uptime, robustness and reliability. Multiple detection options as well as Thermo Scientific™ Viper™ fingertight connections throughout the system…
Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized to for high resolut…
Increase sample throughput for ICP-AES and ICP-MS analysis by reducing sample loading, signal stabilization and washout times
Verex Certified Vial Products: There is a difference! From start to finish, Verex™ CERTIFIED vial and cap products are manufactured to provide high quality, state-of-the-art solutions for your most challenging, sensitive applications. Our innovative precision products offer compatibility with virtually any autosampler and provide air-tight, leak-free seals to safely store and transfer your most important samples. Verex vial…
The DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.
The microDAWN™ determines absolute molecular weight and size of proteins, polymers and other macromolecules in conjunction with UHPLC.
The miniDAWN™ Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering superb sensitivity over a wide range of molecular weight, size and concentration.
The Optilab™ is a unique dRI detector for HPLC/GPC, optimized for use with MALS.
Universal detection for UHPLC and µSEC-MALS.
Enhanced with 20 Years of technology, innovation, and experience, Luna Omega 1.6µm columns build upon the Luna legacy to now provide incredible UHPLC performance and selectivity. With Luna Omega columns you gain: Astounding UHPLC performance and efficiencies Greater separation muscle Better peak shape through an inert foundation Extreme ruggedness and dependability
Rugged C18 with focus on strong hydrophobic retention of non-polar and polar compounds.
100 % aqueous stability and enhanced selectivity/retention for polar analytes without diminishing useful non-polar retention.
One of the World’s Leading Polysaccharide Columns for Chiral Separation
