Luna One of the World's Leading LC Columns

In this application note, the fully porous Luna™ 5 µm C18, 125 x 4.0 mm column was used for the analysis of pantoprazole. Further, modernization of this method was carried out on a fully porous, thermally modified, Luna Omega 3 µm C18, 100 x 3.0 mm column and a fully porous organo-silica Gemini™ 3 µm NX-C18, 100 x 3 mm column. Pantoprazole is a proton pump inhibitor used for the treatment of stomach ulcers and short-term treatment of erosive esophagitis due to gastroesophageal reflux disease (GERD). This study shows the effective separation of pantoprazole from its organic impurities and related substances according to Ph. Eur. Monograph 2296 and USP Monograph for Pantoprazole Sodium Sesquihydrate Organic Impurities Test 2.

In this application note, Phenomenex demonstrates the potential method improvements that can be achieved within the defined allowable adjustments of chromatographic conditions per the USP and Ph. Eur. monographs using Luna™ C18(2) and Kinetex™ C18 columns. Irbesartan is a potent selective angiotensin II type 1 (AT1) receptor antagonist and is used in the treatment of hypertensive patients and diabetic nephropathy. This study for irbesartan and its related substances is based on USP and Ph. Eur monographs where an L1 (USP) or end-capped octadecylsilyl silica gel (Ph. Eur.) stationary phase is used under isocratic conditions.

Explore HPLC column choices and sample preparation options for diverse sample matrices, separation selectivities, and workflows. Download this application note to find the latest word on PFAS analysis - but certainly not the final - on this rapidly expanding field of investigation.

The world is changing and we need to widen our Environmental Scope. Although analytical techniques and established regulated methods are not easily changed; more complex targets, larger lists, and a variety of sample matrices create a daily challenge for environmental chemists. By using the latest technologies and techniques in HPLC/ UHPLC and Sample Preparation, laboratories and chemists are able to develop better approaches to make their work Faster, Cheaper and Easier. Phenomenex is committed to supporting the industry through a comprehensive product portfolio of Sample Preparation, HPLC/UHPLC, and LC-MS, columns and accessories, along with application and method development services to meet industry needs.

This clinical notebook contains a variety of 36 application notes, covering topics in endocrinology and biomarker research, therapeutic drug monitoring, toxicology and sample preparation.

The step-by-step guide gives an overview of the reversed phase HPLC/UHPLC options available. It explains the differences between the range of columns and describes how to choose the right solid support and selectivity for your specific method and goals.

This application note describes the applications of the Luna Omega 1.6 μm column and benefits, including high efficiency and performance, ruggedness and mechanical strength. The article also references other products that can be used in conjunction with the Luna Omega to further optimize your method.

This application note graphically compares a range of Phenomenex® columns by hydrophobicity, steric interaction, hydrogen bonding capacity and cation selectivity. It lists columns that are best suited for separating different types of compounds, such as hydrocarbons and polar basic compounds. It also gives advice on choosing a solid support system.

This application note investigates the use of overlapping SWATH windows to improve the cycle time and reduce the SWATH window size. The ability to acquire all possible MS/MS fragments during each cycle of data acquisition has radically changed peptide quantitation capabilities. Since no prior information is required, data acquisition is greatly simplified. During data processing, the particulars of which peptides and proteins are studied can be changed at any time, without the need for reacquiring any data. This investigation demonstrated that overlapping windows enabled deconvolution to narrower windows without loss in duty cycle and that narrower windows improved MS/MS quality and quantitative properties.

This application note demonstrates how a new preparative Axia column packing process and hardware design, used in conjunction with a reliable, easy-to-use guard column, the SecurityGuard PREP, can improve column lifetime and productivity in high throughput drug discovery labs. The pressure to produce larger amounts of purified drug intermediates and the subsequent drop in sample preparation can result in column decay leading to system shut down and incomplete runs.

In this webinar, Dr. Stephanie Marin, Global Clinical Market Development Manager at Phenomenex, will provide a comprehensive overview of polymeric mixed-mode solid phase extraction (SPE) fundamentals and the properties of polymeric hydrophobic interaction (reversed-phase) SPE sorbents. Attendees will learn how to choose the appropriate SPE phase for their specific compounds of interest, along with an explanation of the ionic functional groups that provide the mixed-mode functionalities, including both weak and strong cation and anion exchange.

Dr. Marin will also delve into the initial extraction conditions required to initiate method development for each type of SPE sorbent, as well as specialized SPE products tailored for drug testing, phospholipid removal, and microelution SPE. This webinar will include numerous clinical research applications to exemplify these concepts.

Key learning objectives

• Discover how to differentiate different polymeric mixed-mode SPE sorbents
• Explore starting conditions for each sorbent type
• Learn how to choose the best SPE sorbent for different analytes
• Understand how to use an SPE method development plate

Who should attend?

• Scientists doing SPE method development
• Labratorians who want to increase their knowledge of polymeric mixed-mode SPE
• Lab managers who want to implement SPE methods in their laboratories

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Discover the benefits of new technology and method development strategies for new techniques