Proteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.
The Protein A and Protein G Quantification Kits extend the Amperia™ Protein Quantification System for streamlined measurement of antibodies across subclasses and species. Using Fc capture with an inverse-occupancy format, the kits deliver reproducible titres directly from crude or clarified samples, with up to 320 tests per kit.
The FRETWorks™ S•Tag™ Assay is a FRET-based method that enables extremely sensitive detection of S•Tag fusion proteins in minutes with a homogeneous format.
ViBE Protein Analysis PlatformPowered by AMMP Technology, the ViBE platform allows rapid design and configuration of protein assays to achieve highly-sensitive detection and quantitation of proteins in complex biological samples with real-time results. The ViBE platform … • Open assay design allows use of your own antibodies and the ability to define sensitivity, dynamic range, and speed requirements for efficient process…
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by…
TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.
The ApopTag ISOL Fluorescence Apoptosis Detection (DNase Types I & II) technique is based upon the biochemical specificity of the enzymes T4 DNA ligase and Vaccinia Virus DNA Topoisomerase I and the unique, dual labeled, dual hairpin oligonucleotide
The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. MitoLight™ partitions differently i…
The CHEMICON In Situ FLICA Pan-Caspase Detection Kit is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
The CHEMICON In Situ FLICA Caspase-3/7 Detection Kit is a fluorescent-based assay for detection of active caspase-3 or caspase-7 in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
The CHEMICON In Situ FLICA Caspase-3 Detection Kit is a fluorescent-based assay for detection of active caspase-3 in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
AXIS devices physically isolate developing neurites from each other and their respective neural cell bodies. In addition, the use of hydrostatic pressure during analysis provides researchers with an opportunity for targeted exposure of neuronal parts to growth factors, drug compounds, or other reagents of biological interest. This ability to selectively control exposure of neuronal outgrowth makes AXIS a powerful platform for…
Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen, such as CB11 (CNBR-digested fragment of type II collagen) (Terato et al, 1985). Antibody-mediated CIA can be induced by i.v. injection of a combination of four different monoclonal antibodies generated by the ArthroMAB…
Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen, such as CB11 (CNBR-digested fragment of type II collagen) (Terato et al, 1985). Antibody-mediated CIA can be induced by i.v. injection of a combination of four different monoclonal antibodies generated by the ArthroMAB…
Collagen-induced arthritis (CIA) in mice is widely used as an experimental model for rheumatoid arthritis (RA) in humans. CIA is mediated by autoantibodies, which bind to a particular region of type II collagen, such as CB11 (CNBR-digested fragment of type II collagen) (Terato et al, 1985). Antibody-mediated CIA can be induced by i.v. injection of a combination of four different monoclonal antibodies generated by the ArthroMAB…
The CHEMICON® QCM™ Chemotaxis 3 μm 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. The 3 μm pore size of this assay's Boyden chambers is appropriate for studyingleukocyte migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples. The Chemicon QCM™ Chemotaxis 3 μm…
The EMD Millipore 5 µm QCM™ Chemotaxis Assay 24-well- colorimetric utilizes a 5 µm pore size, which is appropriate for a subset of fibroblast cells or cancer cells such as NIH-3T3 and MDA-MAB 231 cells. Cells migrated toward chemoattractants were measured by crystal violet, a nucleic dye, and quantified by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random mig…
The CHEMICON® QCM™ Chemotaxis 5 μm 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/macrophage migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples. The Chemicon QCM™ Chemo…
The CHEMICON® QCM™ 24-well Cell Migration Assay (ECM508) eliminates cell pre-labeling and manual counting. The 24-well insert and colorimetric detection format allows for quantitative comparison of multiple samples. The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membran…
The CHEMICON® QCM™ 24-well Cell Migration Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilize…
The Chemicon QCM™ 96-well Migration Assay is ideal for the study of chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 8 μm pore size of this assay's Boyden chambers is appropriate for migration studies of most cell types. Each kit provides sufficient materials for the evaluation of 96 samples. The Chemicon QCM™ 96-well Migration Assay…
The Chemicon QCM™ 96-well 5μm Migration Assay is ideal for studying chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/ macrophage migration. Each kit provides sufficient materials for the evaluation of 96 samples. The Chemicon QCM™ 5μm 96-well Migration…
The Chemicon QCM™ 96-well 3μM Migration Assay is ideal for studying chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 3 μm pore size of this assay's Boyden chambers is appropriate for studying leukocyte migration. Each kit provides sufficient materials for the evaluation of 96 samples. The Chemicon QCM™ 3μM 96-well Migration Assay is i…
