Products & ReviewLife Sciences

Odyssey XF Imaging System

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

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Description

The Odyssey XF uses two NIR lasers, as well as LED and CCD with innovative FieldBrite™ technology for equal illumination across the entire imaging field. Not only will you get more accurate, consistent scans, but you’ll also see the Odyssey XF offers greater sensitivity and dynamic range than film and other CCD imagers. With it, you’ll get the best possible Western blots, chemiluminescent Western blots, nucleic acid gels, and fluorescent protein gels.

The Odyssey XF lays the groundwork for reliable analysis by taking precise, high-integrity images. Gathering all the vital components of separate NIR and chemiluminescent imagers into one, the Odyssey XF is the ideal solution for researchers of all experience levels.

Image your near-infrared (NIR) and enhanced chemiluminescent (ECL) Western blots, detect multiple targets on the same blot with two-channel multiplex fluorescence, and document DNA gels without juggling multiple instruments. Detect your chemiluminescent or NIR Western blots and quantify protein and DNA gels on the same imager. Document your agarose and PAGE gels stained with Ethidium Bromide, SYBR® Safe, SYBR Green I, and many other widely used DNA stains. The patented FieldBrite™ XT2 optics that power the Odyssey XF ensure you get the best image every time.

Capture uniform, low-background images that are not subjected to image correction. Because post-capture image manipulation isn’t required, the Odyssey XF always ensures superior data integrity. Other digital imagers have been known to modify images after capture to correct for non-uniform image brightness or to artificially improve the exposure. However, our patented optics deliver uniform images every time with no post-capture image manipulation. With the Odyssey XF, you can always be confident you have the most accurate, replicable data.

Detect the true detail and complexity of your experimental data. With >6 logs of linear dynamic range, your results are more reliable, and near-infrared fluorescence delivers consistent signals that aren’t affected by time-dependent enzymatic reactions.

To see the whole picture, you need enough dynamic range to consistently document your strongest bands. This allows for accurate comparison within and between labs and experiments. With the Odyssey XF, you’ll capture all your Western blot data (weak and strong) in clear, low-background images every time without adjusting camera settings. Plus, all LICORbio products give you access to an outstanding team of PhD Solutions and Support Scientists.

LICORbio offers unparalleled support from real scientists who understand the high stakes of research. From the first demonstration to post-sale training and support, you’ll have a team you can trust every step of the way. LICORbio instruments are a lasting investment, delivering multi-functional capabilities for decades despite daily use and minimal maintenance.

LICORbio is who scientists turn to when data integrity and clarity of answers matter, because our imagers deliver unmatched dynamic range, sensitivity, and resolution for the highest data fidelity. That’s why our customers choose us again and again.


Application NoteLife Sciences

Good Westerns Gone Bad: Tips to Make Your NIR Western Blot Great

Western blotting is an important tool for the identification of a protein from a complex mixture. This application note from LI-COR highlights the factors that can alter the performance of a Western blot including antibody quality, membrane variability and contamination. A method for accurate Western blot quantification is also presented.


Application NoteLife Sciences

Optimizing Chemiluminescent Western Blots

The Odyssey Fc Imaging System is a CCD-based imager that detects chemiluminescent signal, visible signal at 600 nm, and near-infrared fluorescent signals at 700 nm and 800 nm wavelengths. This technical note highlights both chemiluminescent and near-infrared Western blots using this system. It can detect near-infrared fluorescent markers on a chemiluminescent blot, providing a less expensive alternative to HRP-labeled markers.



White PapersLife Sciences

Maximizing the Performance of Your Western Blot

In this note find tips to make the most of your near-infrared (NIR) Western Blot. Some of the factors, including antibody quality, blocking agent selection, contamination and membrane variability that may alter the performance and imaging of a NIR Western blot are discussed. Methods of maximizing performance using the Odyssey Infrared Imaging System and Odyssey software are also described.


Application NoteLife Sciences

2DE Gel Detection on the Odyssey Infrared Imaging System

The Odyssey Infrared Imaging System provides a sensitive and flexible choice for imaging two-dimensional gel electrophoresis (2DE) gels and blots. Here, LI-COR presents an overview of the 2DE process and viable options for Odyssey detection of proteins separated by 2DE.


Application NoteLife Sciences

Western Blot Analysis

This application note from LI-COR addresses common challenges encountered with Western blotting. Protocols using the Odyssey Systems are described and guidelines are provided for two-colour detection.

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