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Near-Infrared (NIR) Western Blot Detection with the Odyssey Family Imaging Systems

LICORbio
29 Oct 2018

Infrared fluorescence detection with Odyssey Family Imaging Systems provides a quantitative two-color detection method for Western blots. This application note is designed to help you achieve success with NIR Western blot detection methods.

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Odyssey XF Imaging System

LICORbio

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

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Odyssey DLx Infrared Imaging System

LICORbio

The Odyssey DLx Imaging System delivers accurate, reproducible, and quantitative data with near-infrared fluorescence. It supports Western blots, In-Cell Western™ Assays and other plate-based assays, cell-based assays, EMSAs, DNA and protein gels, protein arrays, and tissue section imaging. Discover more with the groundbreaking Odyssey technology cited in over 120,000 peer-reviewed publications.

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Odyssey M Imaging System

LICORbio

The Odyssey M is the most versatile and sophisticated biomolecular imager on the market. With 19 available channels, industry-leading 5 µm resolution, >6 logs of dynamic range, and an onboard luminescence module, the Odyssey M gives researchers a multimodal imaging solution with unparalleled capabilities and performance. Discover more with the groundbreaking Odyssey technology cited in over 40,000 peer-reviewed publications.

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Near Infrared SpectroscopyNear-infrared (NIR) spectroscopy measures the interaction of near-infrared light with a sample, including transmission, reflectance and absorbance, facilitating the identification of analytes. Measurements can be conducted using a Fourier-transform near infrared (FT-NIR) spectrometer, while there are also UV-Vis- NIR spectrophotometers that measure a broader spectrum of wavelengths. Find the best NIR spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Quantitative AnalysesQuantitative analyses involve measuring the amount or concentration of a substance in a sample using analytical techniques. These analyses are essential in fields like chemistry, biology, and environmental science. Explore quantitative analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Blotting

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