Western Blotting Kits
LICORbio Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey infrared imaging systems.
Place your order directly with the manufacturer.
LICORbio Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey infrared imaging systems.
The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes and with either Intercept (PBS) Blocking Buffer or Intercept (TBS) Blocking Buffer. Secondary antibodies in each kit are cross-absorbed for multiplex detection and provide high specificity. Infrared fluorescence detection has changed Western blotting (immunoblotting) by providing unparalleled quantification accuracy and simultaneous two-color multiplexed detection. Simultaneous two-color detection allows exposure of both total protein as well as the amount of protein phosphorylation without stripping and reprobing.
Protein Levels and mRNA Knockdown: The importance of accurate protein quantification in RNA silencing using PCR, Western blotting, and In-Cell Western™ assays.
In this scientific poster, a high quality quantitative analysis of RNA silencing was obtained using two applications of the Odyssey® Infrared Imaging system; the quantitative western blot and the In-Cell Western™ (ICW) assay. Cells transfected with Akt1 siRNA or scrambled siRNA (NS1) were subjected to both quantitative real time PCR (qRT-PCR) and quantitative Western blot analysis.
Quantitative Fluorescent Western Blotting for Protein Analysis in Neurodegenerative Diseases
Dr Tom Wishart from The Roslin Institute, University of Edinburgh, describes the factors involved in neurodegenerative diseases. At the protein level, quantitative fluorescent western blotting is used to analyze neurodegenerative progression as it requires reduced sample concentrations, which can be crucial when working with precious and limited samples such as human patient samples or those from sub-cellular fractionation. Additionally, membranes can be frozen and archived at -80ºC for later re-probing allowing the generation of a membrane based bio-bank for future experimental analyses.
