ZymoBIOMICS™ DNA Mini Kit by Zymo Research

"This extraction kit provided an easy solution to my issues extracting DNA from complex samples with PCR inhibitors. The DNA produced is of excellent yield and quality, and I’ve used it with long-read sequencing to completely assemble multiple genomes."

"Very easy to use. High recovery and good quality DNA."

"Hight effective for metagenome DNA extraction"

"Nice"

"Very effective in studies when extracting dna. Easy to use with clear instructions. "

"Extraction is simple and fast, does not require expensive equipment, and is cheaper than other options on the market. Our microbiome analysis was of high quality, which allowed us to publish in Frontiers in Immunology."

"I use this kit to extract DNA in my metagenomic sequencing projects. In 100% of the cases, I obtain pure DNA from stool samples. Although it's a column extraction, the DNA extracted with this kit is intact (fragment analyser). I especially like that it is ready to use (no need to add solvent to the buffers). Fast and efficient after-sales service."

"The kit was highly useful. economical and gave reproducible results"

"Easy to use and yield is very good for different kind of starting materials."

"I achieved good results by using the ZymoBIOMICS DNA Kit and I was thoroughly helped by it."

"Easy to use and reproducible"

"The obtained yield was very high, and high-quality genomic DNA could also be extracted for 16S Sequencing."

"The kit was easy to use and very effective"

"It’s the simplest and more user-friendly kit that we have in the lab. Easy to use, and with high quality results; even with difficult samples. The price is great and you don’t need to keep it frozen or in complicated conditions. It reduces the time of work compared with other brands."

"We only use Zymo in our lab and this kit is the most effective one! The time of extraction by using this kit is probably 20 minutes and the quality of the DNA is very pure! It's not like the gel purification kit which yields very dirty DNA if you are not careful enough. With this kit, we can trust the DNA quality for cell tranfection."

"An amazing kit which provides the quality, and quantity both. Very useful for the molecular labs and for the DNA extraction procedures. Very happy to have it and use it for my experiments. I will highly recommend it."

"I had excellent repeatability with this kit -testing it on replicates yielded less than 1% difference in any metrics between samples. I was pleased with the ease of incorporating different sample preservatives into the workflow. I'm much happier with this kit than other vendors and won't go back."

"Thank you for Zymo Research. I used the ZymoBIOMICS DNA Kits 2 years ago for metagenome analysis from fecal samples. I am delighted because the quality of DNA that I got is pure, with OD between 1.8 to 2.0. Thank you so much Zymo Research."

"This product gave me good results and was very easy to use as well."

"This product was very useful for the isolation of quality DNA from a variety of microbes. This one kit seemed to work for everything we were isolating and gave really good, clean DNA for DNA sequencing. This is especially helpful in a teaching situation as one kit seems to do it all! The cost is also very attractive woking off of a limited budget. We also use the microbial DNA standards for our metagenomic DNA sequencing runs. Very easy to use and gives consistent results."

"This product easy to use, amazing yield, perfect A260/A280 A230/260"

"The kit was very easy to use and the quality and yield of DNA was very high. The results were reproducible. The technical team helped with customizing the protocol based on our needs."

"The DNA miniprep is our go to product for extractions. In fact whenever we perform extractions we use Zymo kits."

"Worked great for genetic resistance lab!"

"This is the first time I have worked with DNA and this kit was very user friendly. Directions were easy to follow and the steps were well written. The service center was incredibly kind, patient, and helpful with my questions and concerns. I would highly recommend this to any new user or university graduate student wanting to learn DNA extraction."

"It provides reproducible results and resulting DNA is clean when starting material is water"

"Easy to use, reliable and reproducible results."

"The straightforward kit that we found to be comparable with other kits we have used for amplified sequencing analysis (e.g. 16s rRNA targets). Results were consistent with other approaches and students seemed able to complete protocol with the same level of ease as others. The price point is a little better than some other options, but not the best. However, reliability seemed good so definitely, a viable option for us to consider in future projects - I'm always hesitant to switch approaches on existing projects."

"Great product, high quality, reproducible results. Stable shelf life. Versatile!"

"The BIOMICS DNA kit is working great for my compost samples. The general procedure works great, and DNA QC is also good and the results are reproducible."

"The kit comes with easy-to-follow directions and yields useful quantities of clean DNA. Across several sample types and processing runs, I didn't notice any difference in effectiveness or efficiency."

"Achieved high quality results, easy to use."

"This kit is very easy to use and works great for most microbial samples. We use it mostly for bacterial extraction from stool samples. The gDNA collected afterward is great for 16s sequencing or shotgun metagenomics. This is our go to kit for most samples."

"The kit is very easy to use and effective. This is my number 1 kit. Instructions to use are very clear and concise."

"This kit has a great combination of chemicals that can provide good quality and quantity. Easy to use with straightforward protocol to be use. I use it almost everyday and very happy with its performance. Highly recommended."

"This DNA/RNA Kit is not very consistent for the faecal microbiome. RNA output is too low to detect. But it is easy to handle. "

"Quick and efficient microbial DNA recovery kit from complex samples, even those not designed/tested by Zymo. I use this kit to characterise insect microbiota, instead of sample types described in the protocol. But they work nicely, and cheaper than other brands. "

"The workflow for the DNA minikit is straightforward and the columns are easy to use. I always get amazing DNA yields and quality from mouse fecal sample extractions for 16s sequencing and metagenomics analyses. As well, Zymo's product support team is amazing! Would highly recommend this kit for anyone doing gut microbiome research!"

"This kit is very useful for microbial DNA isolation from soil samples. The end product has very high concentration and purity, consistently, and can be directly used in sequencing, making it an ideal tool to have in environmental microbiology molecular studies. As a kit, it is very straight forward to use (5/5), contact with the company regarding one small question was also very easy (5/5) and its cost is adequate for its consistency and quality (5/5)."

"Using the kit was very easy since the DNA extraction is fully optimized. Despite having a low DNA concentration from our samples (as we started from a diluted fecal human sample), we obtained excellent results extracting DNA."

"The ZymoBIOMICS™ DNA Mini Kit provides a cost-effective and reliable tool to isolate DNA from almost any type of sediment - from clear sand to limes heavily loaded with organic matter. Don't waste time on standardization!"

"We got quality results. The kits are user-friendly. Good customer service!"

"It works very well for fungal DNA. However, it’s comparatively less effective against insects."

"Great mini-prep kit. Never had issues with downstream applications using this kit. Preps always came out very clean."

"The product is really good. Can get high quality and yield is enough for regular transfection."

"I have used these kits for my research projects for the last 5 years. They work very well when compared to other microbial DNA kits in the market. The yield and quality of the DNA was enhanced after I started using these kits. The protocol is simple and user friendly. If you have a question, technical customer care is awesome to help. Definitely these kits are value for money and time."

"Great product for extracting DNA from rice leaf tissues."

"I can count on consistent high yields and quality with this kit."

"The extraction kit is robust and easy to use. We used this product and our work has led to a recent publication in Nature (Rao, C., Coyte, K.Z., Bainter, W. et al. Multi-kingdom ecological drivers of microbiota assembly in preterm infants."

"I use this kit for all of my stool extractions and other samples that have a high microbial load. The bead beating is very effective in lysing all of the hard to lyse microbes within or samples. I've found that this kit gives a good representation of the abundance of OTU's within a sample."

"Easy to use, simple instructions to follow for extractions. Quality nucleic acids after extractions that produce great results for Oxford Nanopore next generation sequencing."

"The kit was very easy to follow. I loved the time efficiency of the kit. Other DNA extraction methods take the whole day whereas this is a couple of hours. The DNA worked for what we needed it for. I would purchase again."

"Very easy to use and gives high quality DNA."

"The kit gives DNA of high quality, and the results are reproducible. However, it is sensitive to chitin (for my samples, it is unavoidable), and the yield is twice as low compared to Chelex extraction."

"We are doing DNA-based microbiota analysis in various animal-origin samples, including very low microbial abundance tissue samples. Based on our careful evaluation, this is the best microbial DNA extraction kit especially for this kind of purposes. We are using this for all our microbial DNA extractions."

"I have been using this kit (D4300) to try to extract environmental DNA from swabs. Although I am still working through to find the best procedure/modification to reach my desired DNA yield (> 20 ng/ul), I have been able to increase it from <1 ng/ul to 5-9 ng/ul by following the advices of one of your specialist (Dr. Christian Camacho), who has been incredibly supportive and helpful. I have also appreciated that you sent me some samples of your products to test out."

"Very good kit."

"It worked great for our metagenomics application."

"Best of all, this kit is very easy to use. I was able to successfully obtain fecal DNA for 16S rRNA sequencing. The quality was very good for sequencing and qPCR. However, since the kit contains many reagents, the lids are numbered by me according to the order of use. This will allow you to isolate fecal DNA faster. I've used Zymo Research's product for a long time and have never been disappointed with using them and I'm always satisfied. That's why I rated the ease of use, aftercare, and value for money all 5 stars."

"Compared to other products for similar purposes, this DNA kit provides an economic and efficient way of understanding your own genetic biology, all in one easy to use package."

"This product is excellent for environmental and gDNA. Zymo makes it a very simple and cost effective product. This product serves as the standard in our laboratory and extracting high quality DNA."

"Product worked great compared to other metagenomic DNA prep kits we tried. We got 100 ng/uL yields of DNA and were able to use that DNA directly in subsequent PCR and phi29 reactions for additional amplification. Works great and recommend!"

"This product is very easy to use, reproducible, and great value."

"Steps are easy to follow. Good literature with better sampling method."

"The prep kit is user friendly and decently quick. It gave us more than enough usable DNA for sequencing and validation. Would overall recommend."

"The product was very easy to use and I was able to obtain quality DNA samples from otherwise hard to lyse soil samples."

"The product works great, and I love the $5 gift card that comes with the product. If you order enough it's free shipping too."

"The product is very easy to use and helpful in the NGS workflow."

"Zymo DNA extraction kit is pretty good. Therefore, I am planning to buy DNA cleaning and concentration kit for another experiment. Lets see how it goes."

"Once I learned to cut the environmental water sample filters two directions after curling up (longways and crossways), I got good extractions of our mtDNA. It is not as complicated to use as one other kit I tried and less expensive than a second other kit I used. I also like the fact that we can use the DNA/RNA shield and not have to freeze/thaw the filter samples."

"Easy to use - good purity and yield obtained by undergraduate students"

"Good results, the DNA yield and quality was great!"

"This DNA mini kit is super fast and easy to handle. The DNA quantities as well as the DNA quality are sufficient for many downstream processes. If you want to extract DNA quickly, the kit is just the thing for you."

"Previously, we used self-made buffers and precipitated the DNA with EtOH, which was the cheapest option, but very time-consuming and additional purification of the DNA was necessary for further applications, such as Sanger sequencing. Although small volumes of bacterial suspensions were used, we achieved high amounts of DNA with high purity. Due to the fast and easy to handle protocol the whole procedure of DNA isolation, digestion, and sequencing can now be accomplished within one day. The kit is comparably cheap in addition to the good performance, so we started to frequently use it for testing of bacterial clones."

"The product came with all the applicable columns and reagents needed to complete the process. The price point was good if DNA is the required output - this kit is half the price of the RNA/DNA extraction kit. Clean results run through qPCR without incident."

"We used this product to isolate gDNA from samples in Shield solution. ZymoBIOMICS also have micro prep for low biomass. These products (Shield solution and ZymoBIOMICS) saved my samples and project. The experimental parts of the project are finished. 1 paper published. 1 under review. 1 in-preparation. Thank you Zymo."

"I compared ZymoBIOMICS to QIAGEN and get a better result. My colleague compared 4 kits and found that ZymoBIOMICS kits performed the best."

"The product is widely used by us for the microbiome project. So far this product has not given us any problem. It is easy to use and cost-effective. We got reliable results and published our data in peer-reviewed journals."

"We obtained high quality DNA free of major PCR inhibition using this kit. I thoroughly recommend the kit to anyone wanting DNA suitable for next-generation sequencing applications."

"We had been searching for a kit to extract total DNA from fresh and degrading paint samples for 16S metagenomics sequencing. Most kits available would work with urine, soil and faeces. I then got to know about the ZymoBIOMICS DNA Kit which had a wide sample type coverage though the kit itself had not been tried or tested with paints. Further interactions with the representative in Nigeria on processing the paint samples prior to extraction provided a possible method of getting DNA out of the paint sample. Further optimization of sample amount, sample processing, reagents in the kit and protocol yield good quality and amount of DNA to our amazement. The extracted DNA was subsequently used for 16S metagenomics sequencing and the findings from our work are already been packaged for publication."

"The product is extremely good. The protocol is easy to use and fast. It provides reproducible and high quality DNA applicable for every downstream analysis."

"I had several students use the Zymo kit to extract DNA. In all cases, the extractions were effective."

"Mini Kit works very well. What would be really cool and make life easier, would be if you would color code the vials as well as the tubes columns. So you start with the red solution you load on the red column ... next green and so on. This would reduce the error rate as well increase the speed you can work. Also, the names of the solutions and vials could be simpler."

"As usual, Zymo delivers on an affordable and efficient product that provides a one-step process to extract PCR ready gDNA."

"The Zymo products are all very easy to use and extremely quick. They are well priced compared to their competitors and I find they have a novel approach to simplifying their products overall. I have been impressed with the results so far and would highly recommend people trying their products."

"This kits is easy to use and yielded high quality DNA to send off for sequencing. I can throughly recommend this product to anyone."

"Over all, very pleased with this kit. Looking to purchase more in the coming months."

"The product is easy to use and of good quality. The price is acceptable. Reproducibility is high."

"This kit will reliably extract decent quality DNA from our samples (feces and supernatant). We study mostly gram positive organisms so we use a reliable homogenizer to crack open the cells and have worked with tech support at Zymo to optimize the protocol to get cleaner, higher yields. If you try the kit and find that you aren’t getting the results that you expect, definitely give tech support a call. They have always been very helpful in providing tips to increase quantity and quality of DNA. We have been using this kit for years and appreciate how Zymo updates it when they develop improvements."

"Gives good results for metagenomic extractions. Bashing beads work great for bacteria. We are looking into a magnetic-bead-based extraction to simplify workflow."

"It's an all-inclusive kit for extracting DNA for metagenomics purposes. It works well with the Zymo microbial controls and the hostzero kit. Available in 96 well plates extraction for higher throughput."

"It is easy to use and cost-effective and yield is high."

"Great, easy and reproducible results from a number of environmental isolates."

"Very easy to use, fast and reproducible in my genomic DNA preparations. Reasonably high yields with very clean preps at a very competitive cost."