Member since: 2019
Organization: Institutionen för Medicinskt Cellbiologi
Great instrument for transfections.
Application Area: Transfection/nucleofection
"After struggling with transfection of human embryonic stem cells using other transfection methods, the Lonza 4D nucleofector was a great choice for me. Lonza's 4D nucleofector system is easy to used and easy to optimize the sgRNA concentration, no. of cells and program using the 16 strips cuvette. You can choose different programs in each 16 strips cuvette and it only takes a few seconds for nucleofection. I got high transfection efficiency with high viability. This system is much better than liposome based transfections and other transfection system."
Member since: 2017
Organization: IN CNR Milano
Application Area:Electroporation of neuronal primary cultures
"The Nucleofector is very easy to use but give quite low levels of expression of your construct of interest, at least in neuronal rat primary cultures. Moreover, a very high number of neurons are needed due to the high mortality of the electroporation procedure. In addition, you must use the manufacturer solutions for the procedure, with homemade solutions mortality increases and efficacy decreases. I know there is a new version of the instrument now available and they may ensure better results."
Member since: 2016
Can't imagine doing transfection work without this nucleofector
Application Area:Nucleofection -transfection technology
"This device is wonderful and gives very good results with ES and iPS cells. The equipment is east to use and technical support from the company is fantastic."
Member since: 2012
Organization: Inst Curie
"Our Nuclefector is used to transfect a wide range of cells, ranging from primary cells to cell lines. Nice efficiency for siRNA transfection into both primary cells and cell lines. On the other hand, if electroporation of DNA plasmids is efficient for cells lines, it is rather limited for primary cells (especially neurons and dendritic cells). A bit expensive, but really user friendly."
Member since: 2011
Organization: Institut Curie
"I use the Nuclefector Kit L and have found it to be simple and practical. It is the primary culture of rat cortical neurons. However it is expensive when you have to use for large scale."