STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) is a complete, serum- and animal component-free medium and supplement kit that supports highly efficient differentiation of human ES cells and iPS cells that have been maintained in TeSR™-E8™ to definitive endoderm. Cells differentiated using the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) express high levels of endoderm markers, including CD184 (CXCR4) and SOX17. When used in combination with Vitronectin XF™, the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) provides a completely xeno-free workflow for the generation of multipotent definitive endoderm.
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Manufacturer STEMCELL Technologies Inc.
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