Seahorse XFe24 Analyzers

Uncover the latest recommendations from leaders in immunotherapy research and their insights into exciting new immunotherapy developments. This eBook showcases new analysis tools for scientists developing innovative cell-based solutions.

The Agilent Seahorse XF HS Mini Analyzer enables researchers to profile cellular metabolism with greater flexibility and reliability than ever before, particularly for nonadherent cells or cells with low levels of aerobic metabolism. Researchers can then access these new insights from anywhere, as data generated by this eight-well XF Analyzer are analyzed and interpreted using Seahorse Analytics—a powerful new web-based data analysis application that enables remote, intuitive, Mac- and PC-compatible XF data analysis for all XF Analyzer users.

Agilent Seahorse XF technology delivers real-time insights into cellular energy metabolism, and is relevant to the study of T cell activation and function. This utility is now extended by the Agilent Seahorse XF Hu T Cell Activation Assay, which provides a rapid and standardized method to measure early activation-associated metabolic responses in human T cells, offering important insights into both the early dynamics of activation and its broader metabolic underpinning. The XF Hu T Cell Activation Assay workflow incorporates a soluble CD3/CD28 activator, precoated poly-D-lysine (PDL) XF Cell Culture Microplates, and Agilent Seahorse Analytics, an intuitive, cloud-based data analysis tool.

Alzheimer's disease (AD) is a conformational disease that is characterized by amyloid-β (Aβ) deposition in the brain. Recent reports indicate that Aβ may also interact directly with intracellular proteins such as the mitochondrial enzyme ABAD (Aβ binding alcohol dehydrogenase) in executing its toxic effects. This study employed AG18051, a novel small ABAD-specific compound inhibitor, to investigate the role of ABAD in Aβ toxicity. All data were analyzed using the SeaHorse Bioscience XF24 Analyzer.

This poster describes a method for the metabolic profiling of gynecological cancer cell lines using a Seahorse Bioscience XFe96 Extracellular Flux Analyzer and a novel workstation that enables analysis to be performed under hypoxic conditions as well as air.

This study investigated the metabolic features of immortalized cell lines from different parts of the intestine in comparison to a hepatocyte cell line by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) with the Seahorse Extracellular Flux Analyzer (XF24) under various conditions.

A recent study published by Dohi et al. (2014) examined the effects of molecular hydrogen given in drinking water, using a traumatic brain injury (TBI) animal model and conditionally immortalized mouse cerebral pericyte cells (ImBPC). Dohi et al. used a Seahorse Bioscience XF24 Extracellular Flux Analyzer to measure the changes in mitochondrial respiration using the XF Cell Mito Stress Test and the results of the study are summarized here.

Neurons are particularly reliant on mitochondria for sustaining their high energy demands. Mitochondrial dysfunction has been implicated in neurodegeneration, including in AD. This study aimed to evaluate whether creatine, a documented memory enhancer, influences mitochondrial function. Mitochondrial respiration rates were measured with an XF24 Analyzer from Seahorse Bioscience.

Cellular metabolism is now recognized as a critical mediator of the growth signalling that typifies cell growth and proliferation. This study utilized a novel microplate designed to work with the Seahorse XFe96 Analyzer to assess bioenergetic function in spheroids created from MCF-10A and HCT116 cells.

This study aimed to determine the impact of acute experimentally induced insulin resistance in vivo in the absence of complicating developmental defects. S961 was administered to mice, and the effects on systemic metabolism and gene expression was examined. Oxygen consumption was analyzed using the X24 Bioanalyzer from Seahorse Biosciences.

Join Professor Mercedes Rincon, University of Colorado Anschutz School of Medicine, as she discusses the groundbreaking advancements in adoptive T cell therapy, particularly chimeric antigen receptor (CAR) T cell therapy. While CAR-T therapy has achieved impressive complete remission rates in 60–90% of patients with relapsed/refractory B-cell acute lymphoblastic leukemia (r/r-B-ALL), challenges such as treatment failures and relapses remain significant obstacles.

Metabolism plays a critical role in T cell function, shaping immune responses. While glycolysis is essential for T cell expansion, it can restrict the self-renewal capacity of CAR-T cells during manufacturing. Mitochondrial respiration is vital for their survival and effectiveness. Recent findings have highlighted MCJ, a protein that negatively regulates mitochondrial function in CD8 cells. Loss of MCJ enhances mitochondrial respiration, cytokine secretion, and anti-tumor activity. Targeting MCJ to boost mitochondrial metabolism presents a promising strategy to enhance the efficacy of CAR-T cells and advance adoptive T cell therapies.

Key learning objectives

• Understand the current landscape of adoptive T-cell therapy
• Explain the role of metabolism in T cell function, highlighting the significance of glycolysis and mitochondrial respiration in enhancing CAR-T cell efficacy
• Identify the potential of MCJ as a therapeutic target to improve mitochondrial metabolism, cytokine secretion, and overall effectiveness of CAR-T cell therapies

Who should attend?

• Researchers and scientists specializing in CAR T-cell therapy, immunotherapy development, cell metabolism, glycolysis, and mitochondrial respiration.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Agilent Technologies’ acquisition of BioTek Instruments will strengthen its leadership position in the growing cell analysis segment, says Senior Vice President, Jason Thaysen