Seahorse XFe24 Analyzers by Agilent Technologies

"The Agilent team gave an incredible introduction to the system and how to perform experiments following the purchase of our Ailent Seahorse xfe96, in addition to describing other applications that we had not yet considered. The consumable purchase plan is convenient as well."

"It is easy to use. We got a lot of good data using the equipment, a few high-quality papers have been published."

"The Seahorse XFe96 analyzer was a valuable tool for my research on BMSC cellular metabolism with aging. With the help of user guides and technical support, my mentor and I were able to optimize a protocol and generate useful data. Additionally, the 96-well capacity of this instrument and associated consumables allowed for large numbers of technical replicates with multiple experimental groups on the same plate. The machine and software were easy to use for protocol setup and group labeling, allowing for good record-keeping and data mining during the analysis steps. This is one of the best tools to have in cellular biology and metabolic research!"

"Excellent."

"It is a game-changer in your metabolism assays, it helps you to better understand how mitochondria are working in your cells or tissues."

"Cellular metabolism is a critical factor in stem cell research with recent reports suggesting that both metabolic status and cell culture conditions can significantly alter the differentiation trajectory of stem cells, as well the effect of drugs like chemotherapy and the recovery of cells with stem cells treatment."

"Must for metabolism experiment with clarity in real time."

"There are different models of the Agilent Seahorse available on the market, among them I found the best one is the Agilent Seahorse XFe96 Analyzer because the user can use 96 well plate means to measure the 96 samples in one run. We use the Agilent Seahorse XFe96 to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. The oxygen consumption rate (OCR) and extracellular acidification rates (ECAR) are key indicators of mitochondrial respiration and glycolysis as well as the ATP production rate. This measurement together provides a systems-level view of cellular metabolic function in cultured cells and in vitro samples. The story is not over here, one more powerful software tool is also available in the market like MITOsym from simulation plus. MITOsym uses for the mathematical modeling of the data obtained from the Seahorse XFe analyzer for cellular respiration and bioenergetics. The results obtained from the Seahorse XFe analyzer is very reliable and useful for the development and discovery of new drugs for cancer."

"The Seahorse XFe96 cell analyzer is a benchtop instrument that is mainly intended for our group to analyze mitochondrial function in live cells. It is quite intuitive and the user interface is easy to use with simple directions to operate the analyzer. The data generated is medium throughput and will be adequate for a research group. However, given the cost of consumables from Agilent, researchers may be tempted to use certain reagents sold by other manufacturers."