QIAseq Targeted DNA Pro Panels by QIAGEN
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Status:
Reviewer
Member since: 2023
Organization: Mikrogen Genetic Diseases Center
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- Ease of use
- After sales service
- Value for money
Awesome product to solve lots of NGS problems together.
Application Area: Clinical Research
"The diversity of QIASeq Targeted DNA panel solutions offering kits with appropriate reaction quantities and providing wet laboratory and bioinformatics solutions from isolation to reporting enabled us to provide oncogenetic tests. Especially QIASeq Targeted DNA Pro panels shortens the library preparation duration which is important to shorten turn around time of the tests. Due to unique molecular indices (UMIs) these kits meet specificity, sensitivity, reproducibility criteria. For the implementation of the test in our lab, technical support was quite impressive. In total these kits offer the best performance at their price."
Review date: 10 Apr 2023 | QIAseq Targeted DNA Pro Panels
Status:
Reviewer Member since: 2023
Organization: University of the Philippines
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- Ease of use
- After sales service
- Value for money
One of the best products out in the market!
Application Area:Molecular Biology
"I have always used QIAGEN kits for my research since I was an undergraduate student a decade ago. It’s simple, easy to use, and beginner friendly. They have continued to improve their products and QIAseq Targeted DNA Pro Panels is no exception. This is highly-rated product used by scientists and researchers worldwide!"
Review date: 09 Apr 2023 | QIAseq Targeted DNA Pro Panels
Status:
Reviewer Member since: 2023
Organization: Guidepoint LLC USA
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- Ease of use
- After sales service
- Value for money
Accuracy in the results
Application Area:Sequencing
"Rating 10/10"
Review date: 06 Apr 2023 | QIAseq Targeted DNA Pro Panels
Features
- Less than 6 hours total turnaround time to go from setup to sequencing
- Flexibility to multiplex QIAseq Targeted DNA Pro libraries with other chemistries
- New enzymatic process for preventing adapter and primer carryover contamination, eliminating time-consuming bead purifications
- Reveal structural variants with breakpoints defined at the nucleotide level that span multiple exons, in addition to SNPs and indels
Product Details
QIAseq Targeted DNA Pro Panels set a new standard for NGS genotyping. Major innovations within this new chemistry provide improved ease-of-use, efficiency and expanded variant analysis. This highly optimized DNA target enrichment technology offers an automation-friendly solution that simultaneously facilitates ultrasensitive variant detection using integrated unique molecular indices (UMIs). It is a powerful tool for the detection of genetic variations including somatic mutations, single nucleotide polymorphisms, copy number variation and small insertions/deletions. In addition to our catalog panels, fully customized designs are easily created. Kits are optimized for use with our QIAseq Targeted Pro Panel Unique Dual-Index primers for use with Illumina sequencing systems, as well as Ion-compatible adapters for sequencing on Ion platforms.
Performance
Compatible with diverse sample types including cells, tissue and biofluids, catalog panels target the entire coding region of relevant oncogenes and tumor suppressor genes, as well as actionable and interpretable cancer-related somatic and germline variants.
Building on our expertise employing UMIs to correct for PCR and sequencing errors, coupled with our proprietary highly specific target enrichment, QIAseq Targeted DNA Pro Panels offer a streamlined library preparation workflow that takes less than 6 hours with minimal hands-on time. The simplicity of QIAseq Targeted DNA Pro Panels is suitable for routine known and novel cancer mutation detection in any research laboratory with access to Illumina and Ion Torrent NGS platforms.
Principle
QIAseq Targeted DNA Pro Panels are provided as single-tube primer mixes for a single sequencing reaction with the required amount of template ranging from 10–80 ng for fresh DNA or 100–250 ng for formalin-fixed paraffin-embedded (FFPE) DNA.
Due to the fragmented and modified nature of FFPE DNA samples, many NGS library construction workflows have a low recovery rate from FFPE DNA, whereas QIAseq Targeted DNA Pro incorporates a seamless FFPE DNA repair step before library construction. The specially formulated chemistry achieves highly efficient enrichment even in GC-rich regions. In addition, QIAseq Targeted DNA Pro libraries can be sequenced with Illumina default sequencing primers and are compatible with most mid- to high-throughput Illumina sequencers. Alternative targeted DNA library construction methods have multiple bead cleanup steps that are labor-intensive and result in inconsistent library yield. By replacing the bead cleanup steps with enzymatic processes after the ligation and target enrichment steps, QIAseq Targeted DNA Pro enables a highly efficient and automation-friendly workflow. Data analysis tools have been optimized for seamless variant calling and additional analysis features are supported through QIAGEN Digital Insights solutions.
