Member since: 2023
Organization: Novo Nordisk A/S
Easy to use, fast results, consistency in data produced
Application Area: Day to day cell culture counting; cell numbers & viability
"Pros: Fast & easy cell counting, good assay/instrument for keeping track of suspension cell culture growth (when to split culture and numbers to seed for transfection (VPC2.0 from Thermo-Fisher)) We have standardized when to dilute cell culture (expected cell dens above 2E6/ml always dill 1:10; 50µl ad 500µl) Cons: Sometimes (rare events) counting fails due to camera not able to take pictures? Solution is to close program and restart (but you often "loose" a few Via1 cassettes) Regarding software; would be nice to have the time in data set"
Member since: 2021
One of our major cell culture laboratory device!
Application Area:Cell culture
"Usefull in routine studies (including GLP grade), easy, fast, accurate."
Member since: 2021
Organization: UCL-Biochemical Engineering
Simplifies cell counts, cell cycle analysis and annexin-V assay. Fantastic technology.
Application Area:Cell counts, cell cycle analysis and annexin-V
"Cell counting represents the most basic analytical technique in biotechnology as every single step of a bioprocess relies on knowing the exact cell concentration at the start and at the end. We have been using the NC-3000 (Chemometec) for over 4 years and we are huge fans of it. The system not only allows you to perform cell counts (on suspension cells but also with cells attached to microcarriers) but also cell cycle and annexin-V assays which would normally be performed with flow cytometry. The system stands out in the market as many competitors use purely imaging methods to determine cell counts, viability, and diameter. The NC-3000 (and others from the same company I imagine) use a laser-based system and multiple dyes to detect cell concentration. Our internal validation suggests that the NC-3000 is more precise than alternatives such as manual-based cell counts but also very well known competitors. This would be the first piece of equipment I would purchase when setting up a new lab."
Member since: 2021
Easy-to-use and convenient.
Application Area:Cell counting and transduction efficiency calculations
"The NC-3000 is a robust and easy-to-use cell imaging system, which we use for counting hematopoietic cells and for estimating transduction efficiency. There is a good range of pre-designed assays on the instrument, and the Protocol Adaptation Wizard allows you to easily adapt protocols for your own applications. The 'flow cytometry-like' dot plots are really useful and you can adjust gates accordingly. We mainly use the Via1 cassettes as we don't count numerous samples at the same time, but we are confident in the reproducibility of our data."
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay. With the FlexiCyte™ license, you can create protocols choosing light source, emission filters and detailed settings. The accompanying NucleoView™ software can be used for data analysis of the results and has unlimited licenses.
The NucleoCounter® NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility, and it is calibration-free. The NucleoCounter® NC-3000™ is ideal for the regulated QC, production and even PD and R&D departments. Instrument and method verification tools and 21 CFR Part 11-ready software ensures that the adheres to the requirements and demands of the pharmaceutical and biotech industries. Standardized pdf-reports, user control and registration of audit trails allow for documentation of all procedures. Combined with high precision and limited user influence this makes the NucleoCounter® NC-3000™ a one-of-a-kind cytometer.
Our Field Application Scientists (FASs) provide training, service visits, installation and user guidance on-site or online to ensure you get the best start to working with the NucleoCounter® NC-3000™.