Monolith for binding affinity determination by NanoTemper Technologies

Manufacturer NanoTemper Technologies  |  Available Worldwide
4.9
/
5.0
  |  7 reviews
Monolith uses MST technology to quantify molecular interactions between a target and ligand by detecting changes in fluorescence intensity while a temperature gradient is applied over time.


Monolith for binding affinity determination by NanoTemper Technologies product image
Monolith for binding affinity determination
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.9
7 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: Jagiellonian University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
One of my favorite instruments!
Rating: 4.7

  • Application Area: Proteins

"Easy way to control your protein state. It is less time consuming that other methods."

Review date: 26 Oct 2020 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Purdue University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Super easy to use and maintenance-free!
Rating: 5.0

  • Application Area:Analysis of biomolecule interaction

"This instrument is by far the most sensitive piece of equipment to measure the interaction between small molecule and protein or DNA. It is super easy to use and needs a minimum amount of protein. The control software contains very detailed instruction, and need new users need no training. The instrument is completely maintenance-free. Strongly recommend!"

Review date: 18 Apr 2020 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Department of Biophysics, Semmelweis University, Budapest, Hungary



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
The instrument is indispensable for us to complement our SPR kinetic measurements.
Rating: 5.0

  • Application Area:Protein-protein and protein-lipid interaction studies utilizing recombinant proteins.

"For us it is very important that we are able to measure the affinity of protein interactions from small amount of recombinant proteins, which is a big advantage of the Monolith instrument. The fast, cca 15 min assay time for one titration is a great feature too. We are pretty much satisfied with the company's customer care system, which includes free technical assistance, some on-site demos after purchase, and well-organized user meetings. The company provides help (technical materials and test measurements) to ensure good assay conditions for particular problems. The prices for consumables are acceptable. We also characterize protein interactions via surface plasmon resonance or quartz crystal microbalance, and with the Monolith instrument we can complement those measurements under similar, or even the same, conditions (concentration, pH, buffer composition, etc.). Altogether, the NT.117 is a great instrument, which does not compromise value for price."

Review date: 01 Dec 2016 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: amcure GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
MST made my "affinity days" as a researcher
Rating: 5.0

  • Application Area:Characterization of drug candidates

"Using MST we obtained very convincing data for affinities of small molecules/peptides that were highly reproducible. Experimental set-up is very easy and time-efficient in contrast to some alternative methods. But not only that the technology works reliable, the instruments look extremely aesthetic. Last, but not least, the customer support is engaged and efficient."

Review date: 01 Dec 2016 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: 2bind GmbH



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Nice tool to study drug-target interactions
Rating: 4.7

  • Application Area:Analysis of drug-target interactions

"Our company 2bind uses this technology to study molecular interactions in terms of basic binding parameters. One major focus is on drug-target interactions. The MST offers information on binding affinity, stoichiometry and thermodynamics. The method works in solution with freedom of buffers (even measurements in sera, lysates are possible). It is rapid and consumes very low sample material due to the clever capillary format. We highly appreciate the integrated quality controls showing 1. unspecific adsorption of molecules to the glass surfaces and 2. aggregation effects of the samples. This essential information helps us to optimize the technical setup and obtain optimal data. The output data are highly comparable to ITC and have a good overlap to surface based methods such as SPR. Overall we love this technology, because the size of the interaction partners does not matter. Hence it is the perfect tool to study drug binding to a target protein. The device itself is robust, only takes very little space and the consumables are affordable. The technical support is very good. Thanks guys for your constant support. We can highly recommend this technology."

Review date: 01 Dec 2016 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Centre for chemical Biology and Theraeputics, inStem, NCBS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results, its bread and butter for our lab
Rating: 5.0

  • Application Area:Biomolecular interaction studies

"We use Monolith NT.115 every day for protein and small molecule interaction studies. We developed a single point screen method using this machine, which helps us to screen 100's of compounds every day. Setting up experiments using this machine is very easy and it does not require elaborate training. Consumables are cheap and working cost of the machine is also very low. MO Affinity analysis software is user friendly and using the software is easy and quick."

Review date: 01 Dec 2016 | Monolith for binding affinity determination
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: NIH, NCATS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Rating: 5.0

  • Application Area:Microscale Thermophoresis Detection Of Binding Interactions

"This is a low- to medium-throughput instrument to measure binding constants between two proteins or a protein and a small molecule ligand. The vendor offer two versions: label-based and label-free. Both instrument versions works really well and have consistently produced Kd values close to previously-reported ones or values that end up getting corroborated in the future through traditional calorimetry or SPR techniques. Sample consumption is low and the instrument is very stable (no software crashes or other type breakdown) and easy to use. In our lab, we have used the thermophoresis instruments for over a dozen distinct projects, with results published in almost 10 papers already."

Review date: 12 Oct 2015 | Monolith for binding affinity determination

Knowing the strength of an interaction between key players is important to understand the details behind how biological systems work. With Monolith you have the flexibility to work with all different types of molecules and samples, e.g proteins, small molecules, nucleic acids and more. Especially if you´re having difficulty studying challenging interactions that include membrane proteins, PROTACs, intrinsically disordered proteins (IDPs), or RNA-therapeutics, you´ll need Monolith to characterize them.

Use Monolith to study:

  • Binary binding affinities
  • Ternary interactions that involve three binding partners
  • Binding competition to assess relative affinities of two or more molecules for the same target
  • Binding stoichiometry to calculate the molecular ratios of Binding partners
  • Thermodynamics

Key benefits of working with Monolith:

  • Precisely determine affinity constants (Kds) varying from pM to mM
  • work with only a low amount of sample and use the rest for further experiments
  • choose to work with any buffer that is best for your studied interaction, even in crude lysates or serum

How does it work:

Monolith quantifies molecular interactions free in solution. It uses MST technology to analyze interactions between a target and ligand by detecting changes in fluorescence intensity while a temperature gradient is applied over time. The fluorescent signal comes from the target that is either fluorescently labeled or has intrinsic fluorescence and becomes an extremely sensitive reporter for the interaction. The binding affinity is automatically determined at the end of each run without additional and lengthy data analysis. The Kd is calculated from a fitted curve that plots normalized fluorescence against concentration of ligand.

Product Overview

Monolith for binding affinity determination by NanoTemper Technologies product image

Monolith for binding affinity determination

Manufacturer NanoTemper Technologies  |  Available Worldwide

4.9 / 5.0 | 7 reviews