MitoXpress Xtra Oxygen Consumption Assay by Agilent Technologies

MitoXpress Xtra oxygen consumption assay allows real-time, live cell measurement of extracellular oxygen consumption on fluorescence plate readers.

It is a reliable method to determine respiration rates for metabolic characterization and also to evaluate toxic effects of treatments on mitochondrial function in a high-throughput format.  

Features:  

• Applicable for use with a wide range of in-vitro models:
  • Adherent and suspension cells
  • Permeabilized cells
  • Isolated mitochondria or enzymes
  • Tissues, 3D-cultures and spheroids
  • Bacteria, yeasts and molds
• Assess metabolic flexibility & substrate preference
• Directly evaluate mitochondrial toxicity of treatments
• Perform multiplexing with a range of other assay kits
• Reversible, making transient and fast changes in oxygen consumption observable.

Data analysis, the Agilent Data Visualization Tool is provided to calculate kinetic signal curves and slope values from experimentally-derived fluorescence data.