ÄKTA™ pure™ protein purification system
Flexible and intuitive chromatography system for purification in research applications
Receive your quote directly from the manufacturer.
Overall, the Cytiva ÄKTA system is a powerful and reliable tool for protein purification. Its versatility, precision, and automation capabilities make it an excellent choice for researchers and industrial users alike.
Protein Purification
As a researcher involved in protein purification, I've been using the Cytiva ÄKTA system for several years, and it has proven to be a reliable and versatile tool in my lab. The ÄKTA platform is well-known for its robustness, precision, and ease of use, making it a staple for anyone working in protein purification, from basic research to industrial applications.
Review Date: 9 Aug 2024 | Cytiva
The instrument was a bit confusing at first place but then was easy by knowing pumps
In my university.
I used the instrument as I needed to collect the pure anidbody and antigen complex for my project. In the beginning I faced some problems to set up the suitable buffer but after table shouting I could manage to find the best buffer and correct pH to have the best results out of devices. The instrument was a bit confusing at first place but then when I could imagine how the pumps were working was easy to understand. The purity of antibodies and antigens was very excellent and I didn't lose that much of proteins. The time I spent washing the instrument before and after my work was too long and I could say that was the main problem that it had. Any how the process of washing was mostly hands free so I could deal with it. Another thing in this process was important was the purification coupon which we had to be careful in terms of using and storing
Review Date: 29 Mar 2024 | Cytiva
Can't live without this instrument.
Protein purification
Convenient, easy to use, degeneracy and can handle multiple columns.
Review Date: 5 Feb 2024 | Cytiva
Great results and a workhorse - could imagine life without this instrument!
Protein purification
Simply the best type of protein purification instrument - easily modifiable, and essential for purifying and characterising proteins for downstream applications.
Review Date: 26 Oct 2023 | Cytiva
Works as it should. Always reliable.
Protein purification
It’s an Akta. Does what people expect it will over time. I think they have made it far more user-proof than previous iterations. We’ve had it about 5 years with very few problems.
Review Date: 28 Sept 2023 | Cytiva
Results are reproducible and comparable.
Purification of Peptide API
Software is straightforward to use, results are reproducible and comparable. Service is highly well maintained. Compared to other products in the market slightly expensive but the produced results are robust enough.
Review Date: 16 Jan 2023 | Cytiva
A perfect product with improved chemistry
high salt-based bioreactor output
It was great to observe the results - reduction of HCPs & HCDNAs with maximum loading without any dilutions
Review Date: 13 Sept 2022 | Cytiva
Robust and reliable
Purify proteins
I use the ÄKTA pure from Cytiva, along with a variety of columns attached to it, in order to purify proteins utilizing affinity, ion exchange and affinity chromatography. The machine is in constant use nearly everyday for the past 3 years showing no signs of wear so I would say it is quite robust.
Review Date: 4 Aug 2022 | Cytiva
It is very helpful for downstream process development
Vaccine
After training I can say the system is not so difficult to use. But for working like an average user, you will need at least 1 year of practice. After that it will be easy and you should have a good application expert to answer your problems about both machine and software. With practice and experience from your expert, you can accelerate your learning process.
Review Date: 11 Feb 2022 | Cytiva
Ease of operation and robustness of the equipment.
Purification and chromatography
There is no other such type of instrument still in the market. AKTA instruments are very robust, easy to operate and low maintenance with multi operational functions, all in one instrument. The best part is we can upgrade as per the need by adding more features like extra pump, injection valves etc.
Review Date: 21 Nov 2021 | Cytiva
ÄKTA pure chromatography system allows for quick and easy customization thanks to the modular design of both the hardware and software. Choose from ÄKTA pure 25 with a flow rate up to 25 mL/min or ÄKTA pure 150 with a flow rate up to 150 mL/min and combine with more than 20 hardware options.
How to develop a protein purification strategy
Unlock expert guidance on designing and optimizing protein purification workflows using the Capture, Intermediate Purification, and Polishing (CiPP) strategy. This comprehensive eBook, created in partnership with Cytiva, is your go-to resource for building efficient, scalable purification protocols.
What’s inside the eBook
- A step-by-step breakdown of the CiPP strategy
- Guidance on selecting chromatography techniques based on protein properties
- Real-world application examples for tagged, untagged, and antibody proteins
- Selection guides for prepacked chromatography columns
- Tips for using ÄKTA™ systems and Cytiva resins
- Troubleshooting advice and analytical assay recommendations
Protein purification troubleshooting guide
To resolve your protein purification challenges, explore detailed troubleshooting tips from Cytiva that cover a variety of issues including pressure and flow rate and retention time, to purity and resolution. Gain practical insights to optimize your protein yields and purity, and accelerate your research.
Mag beads: Magnetosphere magazine
In this guide, Cytiva explores the growing demand for high-throughput sample processing and the widespread use of magnetic beads in various applications such as protein purification, next-generation sequencing (NGS), polymerase chain reaction (PCR), and others. Magnetic beads offer automation, reliable performance, and compatibility with diverse sample types, making them increasingly popular. The guide provides insights into surface chemistries for assay development and offers a selection guide. Additionally, it introduces custom services for tailored solutions, allowing modifications to existing beads or the development of new ones to meet specific needs.
Tips for successful purification of histidine-tagged proteins
In this application note, Cytiva provides valuable insights to enhance the outcomes of histidine-tagged protein purification. The note covers reasons for choosing histidine-tagged protein purification, factors affecting protein yield and purity, considerations for tag removal, selecting appropriate chromatography medium, choosing a compatible format, and concludes with key recommendations and support materials.
Guidelines for protein purification at low temperature
Protein purification at low temperatures is crucial for preserving protein integrity and preventing proteolytic degradation, as well as managing protein aggregation tendencies. This application note offers general principles for successful protein purification in cold environments, with ÄKTA™ systems, chromatography resins, and prepacked columns typically performing well at 4°C to 8°C, similar to room temperature results.
How to master automation for your chromatography workflows
Do you need to free up your time in the laboratory? Want to use your ÄKTA™ system to purify samples at a higher throughput while maintaining consistency and accuracy? In this webinar, find out how to improve your chromatography workflows and get ready to automate. Dr. Martin Sichting and Lisa Grufman from Cytiva will discuss how ÄKTA systems can be automated to make your purification process efficient, easy, and reliable.
Key learning objectives
- Learn to combine chromatography steps and run parallel purifications
- Explore affinity chromatography and size exclusion
- Discover how to automate purifications
Who should attend?
- Life science researchers/scientists working with chromatography to purify proteins/molecules.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Customizable automation streamlines protein purification
Discover how ÄKTA™ chromatography systems automate and enhance the efficiency, reproducibility, and scalability of protein purification workflows
Scientists master protein complexity
Experts share insights on protein complexity — offering tools and breakthrough discoveries that empower researchers to overcome obstacles in academia and industry
'SelectScience reviews help guide me in my decision-making process’
Ph.D. student Christopher Argueta shares what inspired his interest in nutrition and why he values science communication and reviews
