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ÄKTA avant chromatography system

Fast and secure development of scalable protein purification methods and processes.

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Average Rating 4.9

|4Scientists have reviewed this product

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Great results, can't live without this instrument.


Average Rating 5.0

Application Area:

Purification and chromatography

Very robust and accurate output data generation. Best in the class in the market. I am using from last 5 years the instrument never disappointed me even once. Very user friendly awith Unicorn Software and automation.

Review Date: 11 Feb 2022 | Cytiva

Very much user friendly and easy to operate.


Average Rating 5.0

Application Area:

Molecular separation and characterisation

Easy to operate, user friendly and up to date as per the needs.

Review Date: 16 Nov 2021 | Cytiva

Great product but not satisfactory after purchase service


Average Rating 4.7

Application Area:

Protein purification

I have been using AKTA FPLC for more than 12 years now. The machine is really easy to use with very interactive interface. The issue comes always if you need to have service. It's hard to get service and also they don't have a good system for following up on the maintenance

Review Date: 18 Jun 2021 | Cytiva

Great instrument.


Average Rating 5.0

Application Area:

Recombinant protein production

One of the best machines you can use for protein purification. Highly versatile for mixing buffer etc.

Review Date: 9 Jun 2021 | Cytiva

Gain efficiency in your process development

ÄKTATM avant is a preparative protein purification chromatography system designed for fast and secure development of scalable methods and processes.

UNICORNTM protein purification software to free up your time

Create methods easily using predefined protocols for a wide range of chromatographic techniques. The integrated design of experiments (DoE) tool helps save time and increase process knowledge. And the UNICORNTM Column Logbook helps you keep track of column usage and performance.

High operational security

A high degree of automation supports operational security when the system is run unattended. The built-in fraction collector keeps your fractions securely cooled when the system is used outside working hours.

Screening and optimization

ÄKTATM avant 25 is optimized for resin screening and method optimization using small HiTrap and HiScreen columns. The integrated BufferPro function as well as multiple sample and buffer inlets add automation possibilities for increased efficiency.


ÄKTATM avant 150 is designed for scaling up to larger columns, such as HiScale and AxiChrom columns, as well as fine-tuning and robustness testing of the optimized process before moving on to the pilot scale. Intelligent Packing of the smallest AxiChrom columns, with 50 and 70 mm inner diameters, is supported via preprogrammed packing methods.

Accelerated process development with DoE

Using the integrated design of experiment (DoE) functionality of UNICORNTM software, the number of experiments required to obtain sufficient process information can be greatly reduced. The software allows you to set up your experimental design and automatically generate chromatography scouting runs to save time.

Quickly prepare your buffers in-line

BufferPro ready-made recipes deliver buffers of desired composition in-line for many pH and conductivity conditions for:

  • automatic scouting
  • preparation of buffer plates for high-throughput process development

Use the quaternary valve to customize buffers for efficient and scalable buffer preparation.

Equipment services

OptiRun service solutions offer a comprehensive range of options that can be customized to your asset-management needs and desired outcomes. Learn more about service agreements and lifecycle support for your equipment.

Product Overview