NTA fast flow histrap column
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Great performance
Protein purification
Great performances: Purification at high yields and with very few contaminants. The best choice for recombinant his-tagged protein purification.
Review Date: 5 Dec 2022 | Cytiva
The yield is very close to the company claims
Microbial biotechnology
It's very easy to use, one step purification set up for recombinant proteins.
Review Date: 13 Feb 2022 | Cytiva
My go-to column for purification of proteins
biomolecules purification
I'm always having his-tagged protein to purify and this is my go-to column. As the column seems to be losing the blue/Ni color quite fast, it's always important to strip and recharge. The column comes with clear instructions on using and recharging.
Review Date: 18 Jun 2021 | Cytiva
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
High binding capacity, approx. 40 mg/mL resin.
Negligible leakage of Ni2+.
Prepacked columns offer reliable and convenient time-saving purification of histidine-tagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.
