KAPA HiFi PCR Kits by Kapa Biosystems, Inc.
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Status:
Expert Reviewer
Member since: 2021
Organization: Strand
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- Ease of use
- After sales service
- Value for money
Kapa - Worth trying it once for your complex custom PCR projects
Application Area: Molecular Biology - PCR
"It's a novel B-family DNA polymerase, specifically engineered to increased it's affinity for DNA without accessing accessory proteins or binding domains. We used to have many custom projects with different gene targets of various sizes and multiple gene panels. We had to switch from 5-6 different polymerase to this Kappa enzyme. Now for most of our projects Kappa is the first and only choice The results are amazing both on gel (quality) and quantity (ND/qubit). The concentration was good enough to use for other downstream applications. You don't need to do many trials while using this polymerase enzyme. It's quite stable and easy to set-up any PCR evading complexity of PCR program. It may be a bit costly but the price is worth for the value of product"
Review date: 04 Mar 2023 | KAPA HiFi PCR Kits
Status:
Reviewer Member since: 2017
Organization: SerImmune Inc
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- Ease of use
- After sales service
- Value for money
Good product, gets the job done.
Application Area:DNA Amplification (PCR)
"We go through several rounds of PCR in the lab and constantly have KAPA on hand. KAPA HotStart ReadyMix has worked great every time. Our Customer service reps, are great and always very helpful. The quality of the product and the service are both good."
Review date: 27 Feb 2019 | KAPA HiFi PCR Kits
Status:
Reviewer Member since: 2017
Organization: Ginkgo Bioworks
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- Ease of use
- After sales service
- Value for money
We rely on it every day and have produced >300,000 NGS samples with it. It's great!
Application Area:NGS Library Prep
"We use the Kapa HiFi PCR mastermix for high-throughput NGS library prep. We prepare 3,000 nextera-based NGS libraries per day, and use Kapa HiFi PCR mastermix for every prep. We have found HiFi to give us the best and most reproducible results with our automated workflow. We have found that kapa hifi is more forgiving in terms of allowing us to run more cycles of pcr without drastically increasing our duplication rate in the NGS data. This allows us to rescue samples on the lower quality or quantity side. Great product!"
Review date: 31 Oct 2018 | KAPA HiFi PCR Kits
KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends.
KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.
Features:
Achieve the highest fidelity
- Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
- The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion
Improve performance on GC- and AT-rich templates
- Achieve higher success rates across targets up to 84% GC content
- Higher yields when amplifying AT-rich templates
Amplify longer targets with greater sensitivity
- High-fidelity PCR of long and complex genomic templates
- Improved sensitivity
- High speed allows significantly shorter reaction times for long range PCR
Applications:
- High-fidelity PCR
- Amplification of DNA fragments for cloning and protein expression or genomic characterization
- Site-directed mutagenesis
- Next-generation sequencing library amplification
Product Overview
